Abed Samer S, P Kiranmayi, Imran Khalid, Lateef Syed S
Department of Biochemistry, Acharya Nagarjuna University, Guntur, IND.
Department of Microbiology, Krupanidhi Degree College, Bengaluru, IND.
Cureus. 2023 Jan 10;15(1):e33585. doi: 10.7759/cureus.33585. eCollection 2023 Jan.
Purpose The present study aimed to determine the antimicrobial nature of juice extract against and to identify its metabolic profile by gas chromatography-mass spectrometry (GC-MS) technique. Materials and methods The cariogenic bacteria were procured from Microbial Type Culture Collection and Gene Bank (MTCC), Chandigarh, India, and revived on brain heart infusion (BHI) agar. The (L.) Osbeck fruits were authenticated from the University of Trans-Disciplinary Health Sciences and Technology (TDU), Bengaluru, India. The antibacterial property of lyophilized lemon juice extract (LJE) dissolved in methanol was evaluated against (MTCC 497) by the agar well diffusion assay. GC-MS technique was employed to identify the volatile metabolite profile of the methanolic LJE sample. The metabolite masses of the respective compounds were identified using the National Institute of Standards and Technology (NIST) library. Results The methanolic LJE sample concentration from 5 to 25 mg/ml did not demonstrate antimicrobial activity, while 30 to 100 mg/ml displayed antibacterial activity against . Chlorohexidine (100 µg/ml) was used as the positive control, while methanol was used as the negative control. Significant antimicrobial metabolites were detected in the methanolic LJE sample by GC-MS analysis. Maleic anhydride, succinic anhydride, 6-Oxa-bicyclo[3.1.0] hexan-3-one, and 3-methyl-2,5-Furandione were the key metabolites identified in the methanolic LJE sample. Conclusion The present study reports that is a potential candidate for the pharmaceutical industry as it possesses bioactive compounds demonstrating therapeutic properties. Further investigations are warranted to determine the individual and synergetic effects of identified metabolites in the methanolic LJE sample for its antimicrobial property. Special attention needs to be focussed on translational research for the development of anti-caries products from .
目的 本研究旨在确定果汁提取物对[具体细菌名称未给出]的抗菌特性，并通过气相色谱 - 质谱联用（GC - MS）技术鉴定其代谢谱。材料与方法 致龋细菌从印度昌迪加尔的微生物模式培养物保藏中心和基因库（MTCC）获取，并在脑心浸液（BHI）琼脂上复苏。[具体水果名称未给出]（L.）Osbeck果实经印度班加罗尔的跨学科健康科学与技术大学（TDU）鉴定。采用琼脂孔扩散法评估溶解于甲醇的冻干柠檬汁提取物（LJE）对[具体细菌名称未给出]（MTCC 497）的抗菌性能。运用GC - MS技术鉴定甲醇提取物LJE样品的挥发性代谢物谱。使用美国国家标准与技术研究院（NIST）库鉴定各化合物的代谢物质量。结果 甲醇提取物LJE样品浓度在5至25 mg/ml时未表现出抗菌活性，而30至100 mg/ml对[具体细菌名称未给出]显示出抗菌活性。氯己定（100 µg/ml）用作阳性对照，甲醇用作阴性对照。通过GC - MS分析在甲醇提取物LJE样品中检测到显著的抗菌代谢物。马来酸酐、琥珀酸酐、6 - 氧杂双环[3.1.0]己烷 - 3 - 酮和3 - 甲基 - 2,5 - 呋喃二酮是在甲醇提取物LJE样品中鉴定出的关键代谢物。结论 本研究报告称[具体水果名称未给出]是制药行业的潜在候选物，因为它含有具有治疗特性的生物活性化合物。有必要进一步研究以确定甲醇提取物LJE样品中鉴定出的代谢物对其抗菌性能的个体和协同作用。在从[具体水果名称未给出]开发抗龋产品的转化研究方面需要给予特别关注。
