Zhan Qi, Thakur Kiran, Feng Jing-Yu, Zhu Yun-Yang, Zhang Jian-Guo, Wei Zhao-Jun
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China; Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, People's Republic of China.
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China.
Food Chem. 2023 Jul 1;413:135656. doi: 10.1016/j.foodchem.2023.135656. Epub 2023 Feb 10.
Recent studies emphasize the improved nutritional and functional status of fermented okara; however, little is known about the metabolite change during fermentation and how it alters metabolic pathways. A metabolomics approach based on untargeted LC-MS reveals metabolic changes in okara fermented by Bacillus subtilis DC-15. We identified 761 differential metabolites, with the highest abundances found in amino acids, dipeptides, fatty acids, small molecule sugars, and vitamins. Moreover, these identified metabolites were mapped to their respective biosynthesis pathways in order to gain a better understanding of the biochemical reactions triggered by fermentation. Based on Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, 485 metabolites were enriched to metabolism-related pathways. They include 37 carbohydrate metabolites, 79 amino acid metabolites, and 22 lipid metabolites. As a result of okara fermentation, we observed a gradual enrichment of metabolites and stabilization of the compounds.
近期研究强调了发酵豆渣改善后的营养和功能状况；然而，关于发酵过程中的代谢物变化以及它如何改变代谢途径，我们所知甚少。一种基于非靶向液相色谱-质谱联用的代谢组学方法揭示了枯草芽孢杆菌DC-15发酵豆渣过程中的代谢变化。我们鉴定出761种差异代谢物，其中氨基酸、二肽、脂肪酸、小分子糖和维生素的丰度最高。此外，这些鉴定出的代谢物被映射到它们各自的生物合成途径，以便更好地理解发酵引发的生化反应。基于京都基因与基因组百科全书（KEGG）分析，485种代谢物富集到与代谢相关的途径。它们包括37种碳水化合物代谢物、79种氨基酸代谢物和22种脂质代谢物。豆渣发酵的结果是，我们观察到代谢物逐渐富集且化合物趋于稳定。
