Mahdavi Morteza, Nanekarani Shahram, Hosseini Seyed Davood
Department of Animal Science, Faculty of Agriculture, Broujerd Branch, Islamic Azad University, Broujerd, Iran.
Razi Vaccine & Serum Research Institute (Arak Branch), Arak, Iran.
Anim Reprod Sci. 2014 Jun 30;147(3-4):93-8. doi: 10.1016/j.anireprosci.2014.04.003. Epub 2014 Apr 13.
The single nucleotide polymorphism of BMPR-IB gene (bone morphogenetic protein receptor type IB) was analyzed using PCR-RFLP in Iranian native Kalehkoohi sheep. BMPR-IB which affects the fecundity of Booroola Merino sheep was studied as a candidate gene associated with the prolificacy of Kalehkoohi sheep. Improving the reproductive traits in sheep could be one of the key factors in increasing farm profitability. Major genes for litter size trait provide opportunities for large and rapid increases in the efficiency of sheep production. The same FecB (Booroola) mutation occurred in the BMPR-IB gene in Kalehkoohi sheep as found in Booroola Merino. Allele frequency for B and + was 0.35 and 0.65 respectively. The BB, B+ and ++ genotypes have been identified with the 0.13, 0.446 and 0.424, respectively. The Kalehkoohi sheep with genotypes BB and B+ had 0.52 and 0.35 lambs, more than the homozygous wild-type, respectively (P<0.01). No significant difference was observed between B+ and BB in litter size. Also the effect of parity and flock weren't significant in this study. Results of the present study support the concept that BMPR-IB significantly affected litter size and was associated with litter size in Kalehkoohi sheep and thus it could be used for Marker-assisted selection programmers for the genetic improvement of reproductive characteristics in this breed.
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术分析了伊朗本土卡莱科希绵羊骨形态发生蛋白受体IB型（BMPR-IB）基因的单核苷酸多态性。作为影响布鲁拉美利奴羊繁殖力的基因，BMPR-IB被作为与卡莱科希羊多产性相关的候选基因进行研究。提高绵羊的繁殖性状可能是提高养殖效益的关键因素之一。产羔数性状的主基因可为大幅快速提高绵羊生产效率提供机会。在卡莱科希绵羊的BMPR-IB基因中发现了与布鲁拉美利奴羊相同的FecB（布鲁拉）突变。B和+等位基因频率分别为0.35和0.65。已分别鉴定出BB、B+和++基因型，频率分别为0.13、0.446和0.424。基因型为BB和B+的卡莱科希绵羊产羔数分别比纯合野生型多0.52只和0.35只（P<0.01）。B+和BB基因型在产羔数上无显著差异。此外，本研究中产次和羊群的影响也不显著。本研究结果支持以下观点：BMPR-IB对卡莱科希绵羊的产羔数有显著影响，并与产羔数相关，因此可用于标记辅助选择程序，以遗传改良该品种的繁殖特性。
