Ranjbar Razie, Zarenezhad Elham, Abdollahi Abbas, Nasrizadeh Marjan, Firooziyan Samira, Namdar Najmeh, Osanloo Mahmoud
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran.
J Trop Med. 2023 Feb 6;2023:5075581. doi: 10.1155/2023/5075581. eCollection 2023.
L. is a widespread medicinal plant with a broad spectrum of biological activity. In the present study, the chemical structure of its essential oil was examined utilizing GC-MS analysis (gas chromatography-mass spectrometry). Then, a nanoemulsion dosage form was prepared with a droplet size and droplet size distribution (SPAN) of 121 ± 3 nm and 0.96. After that, the dosage form of the nanogel was prepared; the nanoemulsion was gelified by the addition of 3.0% carboxymethyl cellulose. In addition, the successful loading of the essential oil into the nanoemulsion and nanogel was approved by ATR-FTIR (attenuated total reflection Fourier transform infrared) analysis. The IC50 values (half maximum inhibitory concentration) of the nanoemulsion and nanogel against A-375 human melanoma cells were 369.6 (497-335) and 127.2 (77-210) g/mL. In addition, they indicated some degrees of an antioxidant activity. Interestingly, after treatment of with 5000 g/mL nanogel, bacterial growth was completely (∼100%) inhibited. In addition, the growth of after treatment with the 5000 g/ml nanoemulsion was decreased by 80%. In addition, nanoemulsion and nanogel LC50 values for larvae were attained as 43.91 (31-62) and 123.9 (111-137) g/mL. Given the natural ingredients and promising efficacy, these nanodrugs can be regarded for further research against other pathogens or mosquito larvae.
L.是一种具有广泛生物活性的药用植物。在本研究中，利用气相色谱 - 质谱联用（GC - MS）分析检测了其精油的化学结构。然后，制备了一种纳米乳剂型，其液滴尺寸和液滴尺寸分布（SPAN）分别为121±3 nm和0.96。之后，制备了纳米凝胶剂型；通过添加3.0%的羧甲基纤维素使纳米乳液凝胶化。此外，衰减全反射傅里叶变换红外光谱（ATR - FTIR）分析证实了精油成功负载到纳米乳液和纳米凝胶中。纳米乳液和纳米凝胶对A - 375人黑色素瘤细胞的半数抑制浓度（IC50）值分别为369.6（497 - 335）和127.2（77 - 210）μg/mL。此外，它们还表现出一定程度的抗氧化活性。有趣的是，用5000μg/mL纳米凝胶处理后，细菌生长被完全（约100%）抑制。此外，用5000μg/ml纳米乳液处理后，[此处原文缺失相关内容]的生长降低了80%。此外，纳米乳液和纳米凝胶对[此处原文缺失相关内容]幼虫的半数致死浓度（LC50）值分别为43.91（31 - 62）和123.9（111 - 137）μg/mL。鉴于其天然成分和有前景的疗效，这些纳米药物可考虑针对其他病原体或蚊虫幼虫进行进一步研究。
