Ziamajidi Nasrin, Daei Sajedeh, Khajvand-Abedini Maryam, Abbasalipourkabir Roghayeh, Nourian Alireza
Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamedan, Iran.
Molecular Medicine Research Center, Hamadan University of Medical Science, Hamedan, Iran.
Chonnam Med J. 2023 Jan;59(1):48-53. doi: 10.4068/cmj.2023.59.1.48. Epub 2023 Jan 25.
Some reports emphasize that zinc oxide nanoparticles (ZnO NPs) are detrimental to the reproductive organs of animals. As such, this research aimed at exploring the apoptotic potential of ZnO NPs on testis along with the beneficial role of Vitamins (V) A, C, and E against ZnO NP-induced damage. To this aim, a population of 54 healthy, male Wistar rats were used in this work and then assigned into nine groups of 6 rats as G1: Control 1 (Water); G2: Control 2 (Olive oil); G3: VA (1000 IU/kg), G4: VC (200 mg/kg), G5: VE (100 IU/kg), G6: ZnO NPs exposed animals (200 mg/kg); and G7, 8 and 9: ZnO NPs-exposed animals that were pre-treated with either VA, C, or E. Apoptosis rates were estimated by measuring the level of apoptotic regulatory markers including Bcl-2-associated X (Bax) and B-cell lymphoma protein 2 (Bcl-2) using western blotting and qRT-PCR assays. The data indicated that ZnO NPs exposure elevates the level of Bax protein and gene expression, whereas the protein and gene expression of Bcl-2 was reduced. Further, the activation of caspase-3,7 occurred after exposure to ZnO NPs, while the above alterations were significantly alleviated in the rats that were co-treated with VA, C, or E and ZnO NPs relative to the rats in the ZnO NPs group. In summary, VA, C, and E exerted anti-apoptotic functions in the testis of rats following administration of ZnO NPs.
一些报告强调，氧化锌纳米颗粒（ZnO NPs）对动物的生殖器官有害。因此，本研究旨在探讨ZnO NPs对睾丸的凋亡潜力，以及维生素（V）A、C和E对ZnO NP诱导损伤的保护作用。为此，本研究使用了54只健康的雄性Wistar大鼠，并将其分为9组，每组6只大鼠，分别为：G1：对照1（水）；G2：对照2（橄榄油）；G3：维生素A（1000 IU/kg）；G4：维生素C（200 mg/kg）；G5：维生素E（100 IU/kg）；G6：暴露于ZnO NPs的动物（200 mg/kg）；以及G7、G8和G9：预先用维生素A、C或E处理过的暴露于ZnO NPs的动物。通过蛋白质免疫印迹法和qRT-PCR检测凋亡调节标志物Bcl-2相关X蛋白（Bax）和B细胞淋巴瘤-2蛋白（Bcl-2）的水平，以评估细胞凋亡率。数据表明，暴露于ZnO NPs会提高Bax蛋白水平和基因表达，而Bcl-2的蛋白和基因表达则降低。此外，暴露于ZnO NPs后会激活caspase-3、7，而相对于ZnO NPs组的大鼠，维生素A、C或E与ZnO NPs联合处理的大鼠中上述变化明显减轻。总之，维生素A、C和E在给大鼠施用ZnO NPs后对睾丸发挥了抗凋亡作用。
