Grube D, Voigt K H, Weber E
Histochemistry. 1978 Dec 28;59(1):75-9. doi: 10.1007/BF00506480.
Semithin and thin serial sections of rat pancreas were investigated by immunocytochemical techniques using antisera against glucagon and endorphin. The immunocytochemical staining methods revealed that in the rat endocrine pancreas endorphin-like immunoreactivity is contained in glucagon (A-) cells. All secretion granules of glucagon cells that could be identified in adjacent thin sections exhibited glucagon as well as endorphin immunoreactivity. Since glucagon cells are devoid of ACTH-like immunoreactivity it is concluded that the biosynthetic pathway of pancreatic endorphin is not the same as in the pituitary.
利用抗胰高血糖素和内啡肽的抗血清，通过免疫细胞化学技术研究了大鼠胰腺的半薄和超薄连续切片。免疫细胞化学染色方法显示，在大鼠内分泌胰腺中，胰高血糖素（A-）细胞含有内啡肽样免疫反应性。在相邻超薄切片中可识别的所有胰高血糖素细胞的分泌颗粒均表现出胰高血糖素和内啡肽免疫反应性。由于胰高血糖素细胞缺乏促肾上腺皮质激素样免疫反应性，因此得出结论，胰腺内啡肽的生物合成途径与垂体中的不同。
