Cetin Y
Histochemistry. 1985;83(4):369-73. doi: 10.1007/BF00684384.
Serial sections from araldite-embedded rat and man pancreata were investigated immunohistochemically for the presence of prodynorphin-related peptides and alpha-endorphin. Immunoreactivities were visualized by the avidin/biotin-peroxidase complex (ABC) technique. In the human pancreas, none of the endocrine cells could be immunostained for prodynorphin-, proopiomelanocortin-related peptides and enkephalins. In the rat pancreas, however, all glucagon cells exhibited immunoreactivities for both beta-neoendorphin and dynorphin A. In addition, these cells contain alpha-endorphin-like immunoreactivity but no immunoreactivities for corticotropin, melanotropin, 16 K-fragment, alpha-N-acetyl-alpha-endorphin and enkephalins. All specificity controls confirmed that the rat endocrine pancreas might be an other source of dynorphin and endorphin with a biosynthetic pathway different from that in the pituitary or in other locations. However, concerning synthesis or degradation of peptide precursor substances interspecies differences may exist.
对包埋在环氧树脂中的大鼠和人类胰腺的连续切片进行免疫组织化学研究，以检测强啡肽原相关肽和α-内啡肽的存在。通过抗生物素蛋白/生物素-过氧化物酶复合物（ABC）技术使免疫反应可视化。在人类胰腺中，没有内分泌细胞能被强啡肽原、促肾上腺皮质激素原相关肽和脑啡肽免疫染色。然而，在大鼠胰腺中，所有的胰高血糖素细胞对β-新内啡肽和强啡肽A均表现出免疫反应性。此外，这些细胞含有α-内啡肽样免疫反应性，但对促肾上腺皮质激素、促黑素、16K片段、α-N-乙酰-α-内啡肽和脑啡肽没有免疫反应性。所有特异性对照均证实，大鼠内分泌胰腺可能是强啡肽和内啡肽的另一个来源，其生物合成途径与垂体或其他部位不同。然而，关于肽前体物质的合成或降解，可能存在种间差异。
