Chen Haozhe, Jiang Nan, Zhang Jie, Tan Peijie, Wang Min, Zhu Songsong, Cao Pinyin
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Oral & Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
ACS Biomater Sci Eng. 2023 Mar 13;9(3):1274-1284. doi: 10.1021/acsbiomaterials.2c01354. Epub 2023 Feb 19.
Three-dimensional (3D) printed implants have attracted substantial attention in the field of personalized medicine, but negative impacts on mechanical properties or initial osteointegration have limited their application. To address these problems, we prepared hierarchical Ti phosphate/Ti oxide (TiP-Ti) hybrid coatings on 3D printed Ti scaffolds. The surface morphology, chemical composition, and bonding strength of the scaffolds were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle measurement, X-ray diffraction (XRD), and scratch test. performance was analyzed by colonization and proliferation of rat bone marrow mesenchymal stem cells (BMSCs). osteointegration of the scaffolds in rat femurs was assessed by micro-CT and histological analyses. The results demonstrated improved cell colonization and proliferation as well as excellent osteointegration obtained by incorporation of our scaffolds with the novel TiP-Ti coating. In conclusion, micron/submicron scaled Ti phosphate/Ti oxide hybrid coatings on 3D printed scaffolds have promising potential in future biomedical applications.
三维（3D）打印植入物在个性化医疗领域引起了广泛关注，但对机械性能或初始骨整合的负面影响限制了它们的应用。为了解决这些问题，我们在3D打印的钛支架上制备了分层的磷酸钛/氧化钛（TiP-Ti）复合涂层。通过扫描电子显微镜（SEM）、原子力显微镜（AFM）、接触角测量、X射线衍射（XRD）和划痕试验对支架的表面形态、化学成分和结合强度进行了表征。通过大鼠骨髓间充质干细胞（BMSCs）的定植和增殖分析其性能。通过微计算机断层扫描（micro-CT）和组织学分析评估支架在大鼠股骨中的骨整合情况。结果表明，通过将我们的支架与新型TiP-Ti涂层结合，细胞定植和增殖得到改善，骨整合效果优异。总之，3D打印支架上的微米/亚微米级磷酸钛/氧化钛复合涂层在未来生物医学应用中具有广阔的潜力。
