State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Disease, and West China Hospital of Stomatology , Sichuan University , Chengdu 610065 , China.
Research Center for Nano-Biomaterials, Analytical and Testing Center , Sichuan University , Chengdu 610065 , China.
ACS Nano. 2018 Aug 28;12(8):7883-7891. doi: 10.1021/acsnano.8b02227. Epub 2018 Jul 10.
In this study, micro/nanoscaled hierarchical hybrid coatings containing titanium (Ti) phosphate and Ti oxide have been fabricated with the aim of promoting osseointegration of Ti-based implants. Three representative surface coatings, namely, micro/nanograss Ti (P-G-Ti), micro/nanoclump Ti, (P-C-Ti), and micro/nanorod Ti (P-R-Ti), have been produced. In-depth investigations into the coating surface morphology, topography, chemical composition, and the surface/cell interaction have been carried out using scanning electron microscopy, transmission electron microscope, X-ray photoelectron spectroscopy, X-ray diffraction, contact-angle measurement, and protein adsorption assay. In addition, in vitro performance of the coating (cell proliferation, adhesion, and differentiation) has been evaluated using rat bone marrow stromal cells (BMSCs), and in vivo assessments have been carried out based on a rat tibia implantation model. All the hybrid coating modified implants demonstrated enhanced protein adsorption and BMSC viability, adhesion and differentiation, with P-G-Ti showing the best bioactivity among all samples. Subsequent i n vivo osseointegration tests confirmed that P-G-Ti has induced a much stronger interfacial bonding with the host tissue, indicated by the 2-fold increase in the ultimate shear strength and over 6-fold increase in the maximum push-out force compared to unmodified Ti implants. The state-of-the-art coating technology proposed for Ti-based implants in this study holds great potential in advancing medical devices for next-generation healthcare technology.
在这项研究中，制备了含有钛磷酸盐和钛氧化物的微/纳尺度分级杂化涂层，旨在促进基于钛的植入物的骨整合。制备了三种代表性的表面涂层，即微/纳米草状钛（P-G-Ti）、微/纳米团簇钛（P-C-Ti）和微/纳米棒状钛（P-R-Ti）。使用扫描电子显微镜、透射电子显微镜、X 射线光电子能谱、X 射线衍射、接触角测量和蛋白质吸附测定对涂层表面形貌、形貌、化学成分以及表面/细胞相互作用进行了深入研究。此外，通过大鼠骨髓基质细胞（BMSCs）评估了涂层的体外性能（细胞增殖、黏附和分化），并基于大鼠胫骨植入模型进行了体内评估。所有杂化涂层修饰的植入物均表现出增强的蛋白质吸附和 BMSC 活力、黏附和分化，其中 P-G-Ti 在所有样品中表现出最佳的生物活性。随后的体内骨整合测试证实，P-G-Ti 与宿主组织之间产生了更强的界面结合，与未修饰的 Ti 植入物相比，其极限剪切强度增加了 2 倍，最大推出力增加了 6 倍以上。本研究中提出的用于 Ti 基植入物的最先进的涂层技术在推进下一代医疗技术的医疗设备方面具有巨大潜力。
