State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Orthognathic and Temporomandibular Joint Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2021 Oct 1;39(5):531-539. doi: 10.7518/hxkq.2021.05.006.
This study was performed to fabricate a bionic coating with titanium (Ti) phosphate to promote the osseointegration of Ti substrate implants.
Phosphorylated micro/nanocoating was prepared on the surface of pure titanium (i.e., TiP-Ti) by hydrothermal process under special pressure, and the untreated smooth pure titanium (cp-Ti) was selected as the control. To evaluate the characteristics of the coating surface, scanning electron microscopy, X-ray diffraction, atomic force microscopy, and contact-angle measurement were performed. In addition, the effects of TiP-Ti on the proliferation, adhesion, and differentiation of rat bone marrow mesenchymal stem cells (BMSCs) were investigated by using cytology. Finally, TiP-Ti implants were implanted into the rat tibia, and the effect of TiP-Ti on the osseointegration in the host was evaluated after 12 weeks.
The TiP-Ti surface presented a bionic structure with coexisting nanoscale 3D spatial structure and microscale pores. experiments showed that the BMSCs had enhanced adhesion, proliferation, and osteogenic differentiation on the TiP-Ti surface. Furthermore, , TiP-Ti showed considerably stronger osseointegration compared with pure titanium, and the ultimate shear strength and maximum pushing force were significantly improved.
A bionic structure with TiP-Ti micro/nanoscale coating was successfully fabricated, indicating a promising method for modifying the surface of implants.
本研究旨在制备具有磷酸钛的仿生涂层，以促进钛基体植入物的骨整合。
通过特殊压力下的水热工艺在纯钛表面制备磷酸化微/纳涂层（即 TiP-Ti），并选择未经处理的光滑纯钛（cp-Ti）作为对照。为了评估涂层表面的特性，进行了扫描电子显微镜、X 射线衍射、原子力显微镜和接触角测量。此外，通过细胞学研究了 TiP-Ti 对大鼠骨髓间充质干细胞（BMSCs）增殖、黏附和分化的影响。最后，将 TiP-Ti 植入大鼠胫骨，并在 12 周后评估 TiP-Ti 对宿主骨整合的影响。
TiP-Ti 表面呈现出具有共存纳米级 3D 空间结构和微尺度孔的仿生结构。实验表明，BMSCs 在 TiP-Ti 表面具有增强的黏附、增殖和成骨分化能力。此外，TiP-Ti 与纯钛相比表现出更强的骨整合能力，最终剪切强度和最大推出力显著提高。
成功制备了具有 TiP-Ti 微/纳尺度涂层的仿生结构，为修饰植入物表面提供了一种有前途的方法。
