Lara Bruna Rossini, de Camargo Bruno Braidotti, Paula Claudete Rodrigues, Monari Gabrielle Pires de Morais, Garces Hans Garcia, Arnoni Mariana Volpe, Silveira Mônica, Gimenes Viviane Mazo Fávero, Leite Junior Diniz Pereira, Bonfietti Lucas Xavier, Oliveira Lidiane, Melhem Márcia de Souza Carvalho, Auler Marcos, Ramos Regina Teixeira Barbieri, Dias Amanda Latercia Tranches, Silva Naiara Chaves, Moreira Débora, Richini-Pereira Virgínia Bodelão, Anversa Laís, Ruiz Luciana da Silva
Instituto de Biociências, Universidade Estadual Paulista (UNESP) , Botucatu, São Paulo, Brazil.
Instituto Adolfo Lutz (IAL)-CLR II, Núcleo de Ciências Biomédicas, Bauru, São Paulo, Brazil.
Med Mycol. 2023 Mar 2;61(3). doi: 10.1093/mmy/myad022.
Trichosporonosis corresponds to a systemic fungal disease that leads to high mortality rates and is frequently associated with medical devices. It affects immunosuppressed patients in particular and is strongly linked to acquired human immunodeficiency, organ and tissue transplants, and malignant hematologic diseases such as leukemia and lymphomas. Trichosporon infections have been increasingly reported worldwide; however, little information is available either about their characteristics or the causative microorganism. Thus, the aims of the present study were: to investigate 59 yeasts of the genus Trichosporon by verifying the biofilm formation capacity of isolates; to analyze the susceptibility patterns of planktonic cells against the antifungals fluconazole, itraconazole, amphotericin-B, voriconazole, and caspofungin by comparing European Committee for Antimicrobial Susceptibility Testing (EUCAST) broth microdilution technique with the commercial method Etest; and to assess the susceptibility patterns of biofilm cells (sessile) against the same antifungals through broth microdilution. The ability to form biofilm on the surface of polystyrene plates was noted for all isolates, and 54.3% of samples were considered strong producers. Comparison between the antifungal susceptibility techniques evidenced that Etest showed higher and discordant minimum inhibitory concentrations (MICs) from those obtained by the microdilution method, especially for fluconazole, itraconazole, and caspofungin. Considering the susceptibility of biofilms, most species had high MIC50 and MIC90 against the tested antifungals, showing 4-to-66-fold higher concentrations for amphotericin B and 2-to-33-fold greater concentrations for caspofungin. These results highlight the importance of further studies with Trichosporon spp. for comparison between laboratory findings and in vivo response, considering both the susceptibility tests and the behavior of biofilm cells against drugs.
毛孢子菌病是一种全身性真菌疾病，死亡率很高，且常与医疗器械相关。它尤其影响免疫抑制患者，与获得性人类免疫缺陷、器官和组织移植以及白血病和淋巴瘤等恶性血液疾病密切相关。全球范围内毛孢子菌感染的报道日益增多；然而，关于其特征或致病微生物的信息却很少。因此，本研究的目的是：通过验证分离株的生物膜形成能力来研究59株毛孢子菌属酵母；通过将欧洲抗菌药物敏感性测试委员会（EUCAST）肉汤微量稀释技术与商业方法Etest进行比较，分析浮游细胞对氟康唑、伊曲康唑、两性霉素B、伏立康唑和卡泊芬净等抗真菌药物的敏感性模式；并通过肉汤微量稀释评估生物膜细胞（固着细胞）对相同抗真菌药物的敏感性模式。所有分离株均具有在聚苯乙烯平板表面形成生物膜的能力，54.3%的样本被认为是强生产者。抗真菌药物敏感性技术之间的比较表明，Etest显示的最低抑菌浓度（MIC）高于且与微量稀释法获得的结果不一致，尤其是对于氟康唑、伊曲康唑和卡泊芬净。考虑到生物膜的敏感性，大多数菌种对测试的抗真菌药物具有较高的MIC50和MIC90，两性霉素B的浓度高出4至66倍，卡泊芬净的浓度高出2至33倍。这些结果凸显了对毛孢子菌属进行进一步研究的重要性，以便在考虑药敏试验以及生物膜细胞对药物的反应的情况下，比较实验室结果与体内反应。
