Buxton T B, Horner J, Hinton A, Rissing J P
Veterans Administration Medical Center, Augusta, Georgia 30910.
J Infect Dis. 1987 Dec;156(6):942-6. doi: 10.1093/infdis/156.6.942.
Osteomyelitic rat tibiae were examined by scanning electron microscopy for the extracellular glycocalyx of Staphylococcus aureus. S. aureus and fractured tibiae from normal rats were incubated together in vitro and examined similarly. Low magnification of endosteal Haversian portals from tibiae studied in vivo and in vitro disclosed adherent S. aureus exuding glycocalyx that buried the organism in dense, coccoid-studded biofilms. The biofilm became progressively more dense over time in vitro and was exuberant at day 70 in vivo. S. aureus incubated in vitro without tibiae disclosed no glycocalyx. Bone chips studied in vitro disclosed staphylococci more commonly near the endosteal Haversian portals than on the intervening endosteal surfaces (mean +/- SE, 280 +/- 75 vs. 12 +/- 3 per 2,500-micron 2 field; P less than .002 by Student's t test). Organisms within ostia were not counted, although they occluded 10%-40% of the ostium. Staphylococci were adherent to exposed woven material, perhaps collagen.
通过扫描电子显微镜检查骨髓炎大鼠胫骨，以观察金黄色葡萄球菌的细胞外糖萼。将金黄色葡萄球菌与正常大鼠的骨折胫骨在体外共同孵育，并进行类似检查。对体内和体外研究的胫骨骨内膜哈弗斯管进行低倍放大观察，发现附着的金黄色葡萄球菌分泌糖萼，使菌体被埋于密集的、布满球菌的生物膜中。随着时间推移，体外生物膜逐渐变得更加致密，在体内70天时生物膜十分旺盛。在无胫骨的情况下体外孵育的金黄色葡萄球菌未发现糖萼。体外研究的骨碎片显示，骨内膜哈弗斯管附近的葡萄球菌比其间的骨内膜表面更常见（平均±标准误，每2500平方微米视野分别为280±75个和12±3个；经学生t检验，P<0.002）。尽管小孔被10%-40%堵塞，但未对小孔内的细菌进行计数。葡萄球菌附着于暴露的编织材料上，可能是胶原蛋白。
