Mayberry-Carson K J, Tober-Meyer B, Smith J K, Lambe D W, Costerton J W
Infect Immun. 1984 Mar;43(3):825-33. doi: 10.1128/iai.43.3.825-833.1984.
A surgical procedure allowed the placement of a silicone rubber catheter in the marrow cavity of the tibia of a rabbit and also allowed the introduction of a sclerosing agent (sodium morrhuate) and cells of Staphylococcus aureus. Osteomyelitis developed in 60% of the animals so treated, and the infecting microorganism was recovered from the infected tibias of the animals that developed this disease. All blood cultures taken 24 h after the infection were negative for S. aureus. Radiological findings consisted of osteolytic changes, the occurrence of sequestration and periosteal reactions, and sclerosis in the infected bones. Sections of bone prepared for histological examination confirmed the diagnosis of osteomyelitis. Transmission and scanning electron microscopy of samples of bone marrow, bone chips, and the catheters taken from the infected tibiae revealed gram-positive cocci embedded in a very extensive matrix of ruthenium red-staining glycocalyx adhering to the bone and the implanted catheter. It is proposed that this extensive glycocalyx served a protective function for the bacteria and was important in bacterial adherence and thus played an important role in bacterial persistence and the development of osteomyelitis in these rabbits.
一种外科手术方法是将硅橡胶导管置于兔子胫骨的骨髓腔内，同时还可引入硬化剂（鱼肝油酸钠）和金黄色葡萄球菌。接受该治疗的动物中有60%发生了骨髓炎，并且从发生此病的动物的感染胫骨中分离出了感染微生物。感染后24小时采集的所有血培养物中金黄色葡萄球菌均为阴性。放射学检查结果包括溶骨性改变、死骨形成和骨膜反应以及感染骨的硬化。为组织学检查制备的骨切片证实了骨髓炎的诊断。对从感染胫骨采集的骨髓、骨碎片和导管样本进行的透射电子显微镜和扫描电子显微镜检查显示，革兰氏阳性球菌嵌入了大量钌红染色的糖萼基质中，这些糖萼附着在骨和植入的导管上。有人提出，这种广泛的糖萼对细菌起到了保护作用，在细菌黏附中起重要作用，因此在这些兔子的细菌持续存在和骨髓炎的发展中发挥了重要作用。
