Li Hang, Fu Shijian, Wang Wenbo
Orthopedics Department, The First Affiliated Hospital of Harbin Medical University, Harbin 150081, Heilongjiang, China.
J Oncol. 2023 Feb 7;2023:9667920. doi: 10.1155/2023/9667920. eCollection 2023.
LncRNA HOTAIR exhibited different effects in human cancers. However, the role of HOTAIR was not reported in osteosarcoma. This study aimed to explore the function of HOTAIR in osteosarcoma. Firstly, we examined HOTAIR expression in breast cancer tissues by the RT-qPCR assay and examined HOTAIR protein expression via immunocytochemistry, to chemical assay, and Western blot. Then, for further exploring the function of HOTAIR, we also examined it by CCK-8 and transwell assays. Downregulation of HOTAIR was detected in osteosarcoma, which predicted poor prognosis of patients with osteosarcoma. Moreover, cell migration, invasion, and proliferation were suppressed by HOTAIR overexpression in osteosarcoma. Furthermore, LPR5 was a direct target of HOTAIR, which was upregulated in osteosarcoma. Especially, the upregulation of LPR5 could impair the suppressive effect of HOTAIR in breast cancer. HOTAIR was found to negatively regulate the EMT and Wnt/-cadherin pathways in osteosarcoma. HOTAIR repressed the progression of osteosarcoma via regulating LPR5 and suppressing the Wnt/-cadherin pathway. Our findings will provide a positive reference for studying the function of HOTAIR in osteosarcoma.
长链非编码RNA HOTAIR在人类癌症中表现出不同的作用。然而，HOTAIR在骨肉瘤中的作用尚未见报道。本研究旨在探讨HOTAIR在骨肉瘤中的功能。首先，我们通过RT-qPCR检测骨肉瘤组织中HOTAIR的表达，并通过免疫细胞化学、化学检测和蛋白质印迹法检测HOTAIR蛋白的表达。然后，为了进一步探究HOTAIR的功能，我们还通过CCK-8和transwell检测进行了研究。在骨肉瘤中检测到HOTAIR表达下调，这预示着骨肉瘤患者预后不良。此外，HOTAIR过表达抑制了骨肉瘤细胞的迁移、侵袭和增殖。此外，LPR5是HOTAIR的直接靶点，在骨肉瘤中上调。特别是，LPR5的上调可能削弱HOTAIR对骨肉瘤的抑制作用。研究发现，HOTAIR在骨肉瘤中对上皮-间质转化和Wnt/β-连环蛋白信号通路起负调控作用。HOTAIR通过调节LPR5和抑制Wnt/β-连环蛋白信号通路抑制骨肉瘤的进展。我们的研究结果将为研究HOTAIR在骨肉瘤中的功能提供积极的参考。
