Siegerist Florian, Drenic Vedran, Koppe Thor-Magnus, Telli Nihal, Endlich Nicole
Institute for Anatomy and Cell Biology, University Medicine Greifswald, Greifswald, Germany.
NIPOKA GmbH, Greifswald, Germany.
Glomerular Dis. 2022 Dec 18;3(1):19-28. doi: 10.1159/000528713. eCollection 2023 Jan-Dec.
For decades, knowledge about glomerular (patho)physiology has been tightly linked with advances in microscopic imaging technology. For example, the invention of electron microscopy was required to hypothesize about the mode of glomerular filtration barrier function.
Super-resolution techniques, defined as fluorescence microscopy approaches that surpass the optical resolution limit of around 200 nm, have been made available to the scientific community. Several of these different techniques are currently in use in glomerular research. Using three-dimensional structured illumination microscopy, the exact morphology of the podocyte filtration slit can be morphometrically analyzed and quantitatively compared across samples originating from animal models or human biopsies.
Several quantitative image analysis approaches and their potential influence on glomerular research and diagnostics are discussed. By improving not only optical resolution but also information content and turnaround time, super-resolution microscopy has the potential to expand the diagnosis of glomerular disease. Soon, these approaches could be introduced into glomerular disease diagnosis.
几十年来，关于肾小球（病理）生理学的知识一直与显微成像技术的进步紧密相连。例如，电子显微镜的发明使得人们能够对肾小球滤过屏障功能的模式进行推测。
超分辨率技术作为一种超越约200纳米光学分辨率极限的荧光显微镜方法，已向科学界开放。目前，肾小球研究中正在使用几种不同的此类技术。利用三维结构光照显微镜，可以对足细胞滤过裂隙的精确形态进行形态计量分析，并对来自动物模型或人类活检的样本进行定量比较。
讨论了几种定量图像分析方法及其对肾小球研究和诊断的潜在影响。通过不仅提高光学分辨率，还提高信息含量和周转时间，超分辨率显微镜有潜力扩大肾小球疾病的诊断范围。很快，这些方法可能会被引入肾小球疾病诊断中。
