运动训练可减轻老年糖尿病前期患者的胰岛素抵抗和肝损伤，与 NLRP3 炎性小体有关。
Exercise training-attenuated insulin resistance and liver injury in elderly pre-diabetic patients correlates with NLRP3 inflammasome.
机构信息
School of Exercise and Health, Shanghai University of Sport, Shanghai, China.
Shanghai Frontiers Science Research Base of Exercise and Metabolic Health, Shanghai University of Sport, Shanghai, China.
出版信息
Front Immunol. 2023 Feb 1;14:1082050. doi: 10.3389/fimmu.2023.1082050. eCollection 2023.
BACKGROUND
Diabetes is one of the most common metabolic diseases and continues to be a leading cause of death worldwide. The NLRP3 inflammasome has been shown to exert detrimental effects on diabetic models. However, evidence linking NLRP3 inflammasome and pre-diabetes has been scarcely explored. Herein, we aimed to determine whether the NLRP3 inflammasome correlates with insulin resistance and liver pathology in a cohort of pre-diabetic subjects.
METHODS
50 pre-diabetic subjects were randomly assigned to a Pre-diabetes Control (DC, n=25) and a Pre-diabetes exercise (DEx, n=25) group. 25 Normal subjects (NC) were selected as controls. The DEx group performed a 6-month combined Yijingjing and resistance training intervention, while DC and NC group remained daily routines. Clinical metabolic parameters were determined with an automatic biochemistry analyzer; inflammatory cytokines were quantified by the ELISA assay; the protein expressions of NLRP3 inflammasome components in PBMCs were evaluated by Western Blot.
RESULTS
The insulin resistance, liver injury and NLRP3 inflammasome activity were higher in pre-diabetic individuals than in normal control group. However, 6-month exercise intervention counteracted this trend, significantly improved insulin sensitivity, reduced liver injury and inhibited the overactivation of NLRP3 inflammasome in pre-diabetic subjects. Moreover, positive correlations between insulin resistance, liver pathology and NLRP3 inflammasome were also found.
CONCLUSIONS
Our study suggests that exercise training is an effective strategy to alleviate insulin resistance and liver injury in elderly pre-diabetic subjects which is probably associated with the inhibition of NLRP3 inflammasome activity.
背景
糖尿病是最常见的代谢疾病之一，仍然是全球范围内主要的死亡原因。NLRP3 炎性小体已被证明对糖尿病模型具有有害影响。然而，与 NLRP3 炎性小体和前驱糖尿病相关的证据几乎没有被探索过。在此，我们旨在确定 NLRP3 炎性小体是否与前驱糖尿病患者的胰岛素抵抗和肝脏病理相关。
方法
50 名前驱糖尿病患者被随机分为前驱糖尿病对照组（DC，n=25）和前驱糖尿病运动组（DEx，n=25）。选择 25 名正常受试者（NC）作为对照组。DEx 组进行了 6 个月的易经经和抗阻训练联合干预，而 DC 和 NC 组保持日常活动。使用自动生化分析仪测定临床代谢参数；通过 ELISA 测定炎性细胞因子；通过 Western Blot 评估 PBMCs 中 NLRP3 炎性小体成分的蛋白表达。
结果
与正常对照组相比，前驱糖尿病患者的胰岛素抵抗、肝损伤和 NLRP3 炎性小体活性更高。然而，6 个月的运动干预逆转了这种趋势，显著改善了胰岛素敏感性，减轻了肝损伤，并抑制了前驱糖尿病患者 NLRP3 炎性小体的过度激活。此外，还发现了胰岛素抵抗、肝病理和 NLRP3 炎性小体之间的正相关关系。
结论
我们的研究表明，运动训练是一种有效的策略，可以减轻老年前驱糖尿病患者的胰岛素抵抗和肝损伤，这可能与抑制 NLRP3 炎性小体活性有关。
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