Ibrahim G F, Lyons M J, Walker R A, Fleet G H
Appl Environ Microbiol. 1985 Sep;50(3):670-5. doi: 10.1128/aem.50.3.670-675.1985.
Radioimmunometric and enzyme-immunometric assays were developed for the detection of salmonellae in pure and mixed cultures as well as in 59 food samples. The performances of titanous hydroxide suspension and microtiter plates as the solid phase for the immobilization of microorganisms were compared in these immunoassays. Detection of populations of salmonella cells in pure culture, diluted with saline, was 4- to 10-fold more sensitive with the microtiter plates. However, with mixed culture of salmonella and other enterobacterial species, the detection sensitivity with titanous hydroxide was 100- to 160-fold more sensitive than with microtiter plates. Good correlation existed between results of a standard cultural method for the detection of salmonellae in foods and those obtained from radioimmunometric and enzyme-immunometric assays utilizing titanous hydroxide. However, a high incidence of false-positive and false-negative results with food samples occurred with the enzyme-immunometric assay utilizing microtiter plates. The results provided strong evidence for the merits of substituting titanous hydroxide for microtiter plates as the solid phase for the immobilization of salmonellae for their detection by immunoassays. The immunoassays were rapid and enabled the analysis of a large number of selective enrichment cultures of food samples for salmonellae within 8 h.
已开发出放射免疫测定法和酶免疫测定法，用于检测纯培养物和混合培养物以及59份食品样本中的沙门氏菌。在这些免疫测定中，比较了氢氧化钛悬浮液和微量滴定板作为固定微生物的固相的性能。对于用盐水稀释的纯培养沙门氏菌细胞群体的检测，微量滴定板的灵敏度要高4至10倍。然而，对于沙门氏菌与其他肠杆菌属的混合培养物，氢氧化钛的检测灵敏度比微量滴定板高100至160倍。食品中沙门氏菌检测的标准培养方法结果与使用氢氧化钛的放射免疫测定法和酶免疫测定法结果之间存在良好的相关性。然而，使用微量滴定板的酶免疫测定法对食品样本的假阳性和假阴性结果发生率很高。结果提供了有力证据，证明用氢氧化钛替代微量滴定板作为固定沙门氏菌的固相用于免疫测定法检测的优点。这些免疫测定法速度快，能够在8小时内对大量食品样本的沙门氏菌选择性富集培养物进行分析。
