Wang Bing, Zhou Xiang, Liu Wei, Liu Mei-Han, Mo Dan, Wu Qing-Feng, Wang Ya-Juan, Zhang Miao-Miao, Chen Lei, Yuan Shan, Zhou Bo, Li Xin, Lu Dong
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, China.
Front Microbiol. 2023 Feb 7;14:1065953. doi: 10.3389/fmicb.2023.1065953. eCollection 2023.
has considerable prospect in the production of organic acids. Globally, refinery final molasses is rich in sugar and reported to have high levels of accumulation and high emission costs, recognized as an excellent substrate for fermentation, but there is no suitable method available at present.
In this study, an acid-base treatment combined with a new green membrane treatment technology - a dynamic ion-exchange membrane -was used to pretreat refinery final molasses, so that it could be used for to produce butyric acid. A high-performance liquid chromatography method was established to determine the conversion of a large amount of sucrose into fermentable sugars (71.88 g/L glucose and 38.06 g/L fructose) in the treated refinery final molasses. The process of sequential filtration with 3, 1, and 0.45 μm-pore diameter dynamic ion-exchange membranes could remove impurities, pigments, and harmful substances from the refinery final molasses, and retain the fermentable sugar.
This means that refinery final molasses from the sugar industry could be utilized as a high-value by-product and used for the growth of , with industrial feasibility and economic competitiveness. Using the treated refinery final molasses as a carbon source, was screened by the method of adaptive evolution. The strain with butyric acid yielded 52.54 g/L, and the yield of the six carbon sugar was increased from 0.240 to 0.478 g/g. The results showed that combination of and ionic membrane technology broke through the bottleneck of its utilization of refinery final molasses. This study provided an innovative idea for the fermentation to produce butyric acid.
在有机酸生产方面具有可观的前景。在全球范围内，炼厂最终糖蜜富含糖分，据报道具有高积累水平和高排放成本，被认为是发酵的优良底物，但目前尚无合适的方法。
在本研究中，采用酸碱处理结合一种新的绿色膜处理技术——动态离子交换膜，对炼厂最终糖蜜进行预处理，使其可用于生产丁酸。建立了一种高效液相色谱法，以测定经处理的炼厂最终糖蜜中大量蔗糖转化为可发酵糖（71.88 g/L葡萄糖和38.06 g/L果糖）的情况。用孔径为3、1和0.45μm的动态离子交换膜依次过滤的过程可去除炼厂最终糖蜜中的杂质、色素和有害物质，并保留可发酵糖。
这意味着制糖工业的炼厂最终糖蜜可作为高价值副产品加以利用，并用于[具体微生物名称]的生长，具有工业可行性和经济竞争力。以经处理的炼厂最终糖蜜为碳源，通过适应性进化方法筛选出[具体微生物名称]。该菌株丁酸产量为52.54 g/L，六碳糖产量从0.240提高到0.478 g/g。结果表明，[具体微生物名称]与离子膜技术的结合突破了其利用炼厂最终糖蜜的瓶颈。本研究为[具体微生物名称]发酵生产丁酸提供了创新思路。
