利用固定化酪丁酸梭状芽孢杆菌在纤维床生物反应器中从甘蔗糖蜜进行丁酸发酵。
Butyric acid fermentation in a fibrous bed bioreactor with immobilized Clostridium tyrobutyricum from cane molasses.
作者信息
Jiang Ling, Wang Jufang, Liang Shizhong, Wang Xiaoning, Cen Peilin, Xu Zhinan
机构信息
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, PR China.
出版信息
Bioresour Technol. 2009 Jul;100(13):3403-9. doi: 10.1016/j.biortech.2009.02.032. Epub 2009 Mar 17.
Butyrate fermentation by immobilized Clostridium tyrobutyricum was successfully carried out in a fibrous bed bioreactor using cane molasses. Batch fermentations were conducted to investigate the influence of pH on the metabolism of the strain, and the results showed that the fermentation gave a highest butyrate production of 26.2 g l(-1) with yield of 0.47 g g(-1) and reactor productivity up to 4.13 g l(-1)h(-1) at pH 6.0. When repeated-batch fermentation was carried out, long-term operation with high butyrate yield, volumetric productivity was achieved. Several cane molasses pretreatment techniques were investigated, and it was found that sulfuric acid treatment gave better results regarding butyrate concentration (34.6+/-0.8 g l(-1)), yield (0.58+/-0.01 g g(-1)), and sugar utilization (90.8+/-0.9%). Also, fed-batch fermentation from cane molasses pretreated with sulfuric acid was performed to further increase the concentration of butyrate up to 55.2 g l(-1).
利用甘蔗废蜜，在纤维床生物反应器中成功实现了固定化酪丁酸梭菌的丁酸盐发酵。进行了分批发酵以研究pH对该菌株代谢的影响，结果表明，在pH 6.0时，发酵产生的丁酸盐最高产量为26.2 g l(-1)，产率为0.47 g g(-1)，反应器生产率高达4.13 g l(-1)h(-1)。进行重复分批发酵时，实现了丁酸盐高产率、体积生产率的长期运行。研究了几种甘蔗废蜜预处理技术，发现硫酸处理在丁酸盐浓度（34.6±0.8 g l(-1)）、产率（0.58±0.01 g g(-1)）和糖利用率（90.8±0.9%）方面效果更好。此外，对经硫酸预处理的甘蔗废蜜进行补料分批发酵，将丁酸盐浓度进一步提高至55.2 g l(-1)。
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