Liu Tingting, Zhu Liying, Zhu Zhengming, Jiang Ling
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 210009, People's Republic of China.
College of Chemical and Molecular Engineering, Nanjing Tech University, Nanjing, 210009, People's Republic of China.
Curr Microbiol. 2020 Nov;77(11):3685-3694. doi: 10.1007/s00284-020-02175-0. Epub 2020 Sep 4.
Clostridium tyrobutyricum is a promising microbial host for the anaerobic production of bio-based chemicals, especially butyric acid. At the same time, it also has great potential as a probiotic for the production of short-chain fatty acids in the intestines. However, due to the insufficient knowledge of the genetic characteristics of this organism, there has been little progress in its genetic engineering to date. Here, we present the complete genome sequence of C. tyrobutyricum L319, which consists of a circular chromosome and a plasmid with a G + C content of 31.69%, encompassing approximately 3.09 Mb with 3052 protein-coding genes. Functional gene annotation revealed better results than previous studies based on KEGG pathway classification. Furthermore, we obtained detailed functional characterization of 93 genes previously annotated as putative proteins. Genomic analysis revealed that this organism contains multiple genes encoding enzymes involved in the CRISPR-Cas systems, substrate utilization, isopeptide and ester bonds, transcriptional regulation, and oxidative stress. The safety evaluation at genetic level indicated that this organism does not possess transferable resistance genes, invasive defensive pathogenicity factors, or harmful enzymes. The genome sequence data analyzed in this study will be available for further research and will facilitate the further understanding and development of potential applications of C. tyrobutyricum.
酪丁酸梭菌是一种很有前景的微生物宿主，可用于厌氧生产生物基化学品，尤其是丁酸。同时，它作为一种益生菌在肠道中生产短链脂肪酸也具有巨大潜力。然而，由于对该生物体遗传特性的了解不足，迄今为止其基因工程进展甚微。在此，我们展示了酪丁酸梭菌L319的完整基因组序列，它由一个环状染色体和一个质粒组成，G+C含量为31.69%，约3.09 Mb，包含3052个蛋白质编码基因。功能基因注释显示，与基于KEGG通路分类的先前研究相比，结果更好。此外，我们还获得了93个先前注释为假定蛋白质的基因的详细功能特征。基因组分析表明，该生物体含有多个编码参与CRISPR-Cas系统、底物利用、异肽和酯键、转录调控以及氧化应激的酶的基因。遗传水平的安全性评估表明，该生物体不具有可转移的抗性基因、侵袭性防御致病因子或有害酶。本研究中分析的基因组序列数据将可供进一步研究，并将有助于进一步了解和开发酪丁酸梭菌的潜在应用。
