Department of Chemistry, The University of Texas at Austin, Austin, TX 78712, USA.
Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.
J Mater Chem B. 2023 Jun 21;11(24):5416-5428. doi: 10.1039/d2tb02825e.
Bioactive hydrogel coatings offer a promising route to introduce sustained thromboresistance to cardiovascular devices without compromising bulk mechanical properties. Poly(ethylene glycol)-based hydrogels provide antifouling properties to limit acute thromobosis and incorporation of adhesive ligands can be used to promote endothelialization. However, conventional PEG-based hydrogels at stiffnesses that promote cell attachment can be brittle and prone to damage in a surgical setting, limiting their utility in clinical applications. In this work, we developed a durable hydrogel coating using interpenetrating networks of polyether urethane diacrylamide (PEUDAm) and poly(-acryloyl glycinamide) (pNAGA). First, diffusion-mediated redox initiation of PEUDAm was used to coat electrospun polyurethane fiber meshes with coating thickness controlled by the immersion time. The second network of pNAGA was then introduced to enhance damage resistance of the hydrogel coating. The durability, thromboresistance, and bioactivity of the resulting multilayer grafts were then assessed. The IPN hydrogel coatings displayed resistance to surgically-associated damage mechanisms and retained the anti-fouling nature of PEG-based hydrogels as indicated by reduced protein adsorption and platelet attachment. Moreover, incorporation of functionalized collagen into the IPN hydrogel coating conferred bioactivity that supported endothelial cell adhesion. Overall, this conformable and durable hydrogel coating provides an improved approach for cardiovascular device fabrication with targeted biological activity.
生物活性水凝胶涂层为心血管设备提供持续抗血栓的方法，在不影响整体机械性能的前提下，具有广阔的应用前景。基于聚乙二醇（PEG）的水凝胶具有抗污性能，可限制急性血栓形成，而黏附配体的掺入可促进内皮化。然而，在促进细胞附着的刚度下，传统的基于 PEG 的水凝胶可能会很脆，在手术环境中容易受损，限制了它们在临床应用中的实用性。在这项工作中，我们使用聚醚型聚氨酯二丙烯酰胺（PEUDAm）和聚（丙烯酰基甘氨酰胺）（pNAGA）的互穿网络开发了一种耐用的水凝胶涂层。首先，通过扩散介导的氧化还原引发作用，将 PEUDAm 涂覆到静电纺丝的聚氨酯纤维网格上，涂层厚度通过浸渍时间来控制。然后引入第二个 pNAGA 网络，以增强水凝胶涂层的抗损伤能力。然后评估了所得多层接枝体的耐久性、抗血栓性和生物活性。互穿网络水凝胶涂层表现出对与手术相关的损伤机制的抵抗力，并且保留了基于 PEG 的水凝胶的抗污性质，表现为蛋白质吸附和血小板附着减少。此外，将功能化胶原蛋白掺入互穿网络水凝胶涂层中赋予了生物活性，支持内皮细胞黏附。总的来说，这种顺应性和耐用性的水凝胶涂层为心血管设备的制造提供了一种改进的方法，具有靶向的生物活性。
