Instituto Federal de Educação, Ciência e Tecnologia de Pernambuco (IFPE), Campus Barreiros, 55560-000 Recife, PE, Brazil.
Industrial Biotechnology and Systems Biology Research Group (IBSB), Department of Bioengineering, Marmara University, Istanbul, Turkey.
Int J Biol Macromol. 2023 Apr 30;235:123800. doi: 10.1016/j.ijbiomac.2023.123800. Epub 2023 Feb 23.
Levan is a homopolysaccharide of fructose that has both scientific and industrial importance, with various applications in health, pharmaceutical, cosmetic and food industries. Despite its broad spectrum of uses, there are only a limited number of commercial levan sources due to the high costs related to its production. To make production economically viable, efforts have been concentrated on the selection of levan-producing microorganisms, the genetic manipulation of new strains, and the use of inexpensive agro-industrial byproducts as substrates. Another efficient strategy involves the concomitant synthesis of other products with high market value and as such, the successful co-production of levan was demonstrated with fructooligosaccharides, ethanol, sorbitol, poly-ε-lysine, poly-γ-glutamic acid and polyhydroxyalkanoates. This paper offers a systematic review of important aspects regarding recent strategies involving the simultaneous synthesis of levan and other bioproducts of aggregate value reported to date and discusses the challenges and opportunities for its large-scale production and applications.
寡糖是一种由果糖组成的同多糖，具有重要的科学和工业价值，在健康、制药、化妆品和食品工业中有多种应用。尽管它的用途广泛，但由于其生产成本高，商业寡糖的来源有限。为了使生产具有经济可行性，人们集中精力从生产寡糖的微生物的选择、新菌株的遗传操作以及使用廉价的农业工业副产品作为基质等方面进行努力。另一个有效的策略是同时合成其他具有高市场价值的产品，因此，已经成功地与低聚果糖、乙醇、山梨醇、聚ε-赖氨酸、聚γ-谷氨酸和聚羟基烷酸等共同生产了寡糖。本文对迄今为止报道的同时合成寡糖和其他具有综合价值的生物制品的最新策略的重要方面进行了系统综述，并讨论了其大规模生产和应用的挑战和机遇。
