Amity Institute of Biotechnology, Amity University Maharashtra, Mumbai-Pune Expressway, Bhatan, Panvel, Mumbai 410206, Maharashtra, India.
Amity Centre of Excellence in Astrobiology, Amity University Maharashtra, Mumbai-Pune Expressway, Bhatan, Panvel, Mumbai 410206, Maharashtra, India.
Molecules. 2023 Sep 25;28(19):6797. doi: 10.3390/molecules28196797.
Sugar carbonyl groups interact with protein amino groups, forming toxic components referred to as advanced glycation end products (AGEs). The glycation system (BSA, a model protein, and fructose) was incubated for five weeks at 37 °C in the presence and absence of Stevia leaf extract. The results indicated that the leaf extract (0.5 mg/mL) decreased the incidence of browning (70.84 ± 0.08%), fructosamine (67.27 ± 0.08%), and carbonyl content (64.04 ± 0.09%). Moreover, we observed an 81 ± 8.49% reduction in total AGEs. The inhibition of individual AGE (argpyrimidine, vesper lysine, and pentosidine) was ~80%. The decrease in the protein aggregation was observed with Congo red (46.88 ± 0.078%) and the Thioflavin T (31.25 ± 1.18%) methods in the presence of Stevia leaf extract. The repercussion of Stevia leaf extract on DNA glycation was examined using agarose gel electrophoresis, wherein the DNA damage was reversed in the presence of 1 mg/mL of leaf extract. When the HDF cell line was treated with 0.5 mg/mL of extract, the viability of cells decreased by only ~20% along with the same cytokine IL-10 production, and glucose uptake decreased by 28 ± 1.90% compared to the control. In conclusion, Stevia extract emerges as a promising natural agent for mitigating glycation-associated challenges, holding potential for novel therapeutic interventions and enhanced management of its related conditions.
糖羰基与蛋白质氨基相互作用，形成被称为晚期糖基化终产物（AGEs）的有毒成分。在 37°C 下，将糖化系统（BSA、模型蛋白和果糖）与甜菊叶提取物一起孵育五周。结果表明，叶提取物（0.5mg/mL）降低了褐变（70.84±0.08%）、果糖胺（67.27±0.08%）和羰基含量（64.04±0.09%）的发生率。此外，我们观察到总 AGE 减少了 81±8.49%。对单个 AGE（精氨酰嘧啶、vesper 赖氨酸和戊糖素）的抑制作用约为 80%。在存在甜菊叶提取物的情况下，用刚果红（46.88±0.078%）和硫代黄素 T（31.25±1.18%）方法观察到蛋白质聚集的减少。使用琼脂糖凝胶电泳检查了甜菊叶提取物对 DNA 糖化的影响，在 1mg/mL 叶提取物存在下，DNA 损伤得到逆转。当 HDF 细胞系用 0.5mg/mL 的提取物处理时，细胞活力仅下降了约 20%，同时产生相同的细胞因子 IL-10，葡萄糖摄取量比对照组下降了 28±1.90%。总之，甜菊叶提取物作为一种有前途的天然药物，可以减轻糖基化相关的挑战，为新的治疗干预措施和更好地管理其相关疾病提供了潜力。
