Miłek Michał, Ciszkowicz Ewa, Sidor Ewelina, Hęclik Joanna, Lecka-Szlachta Katarzyna, Dżugan Małgorzata
Department of Chemistry and Food Toxicology, Institute of Food Technology and Nutrition, University of Rzeszów, Ćwiklinskiej 1a, 35-601 Rzeszów, Poland.
Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland.
Antibiotics (Basel). 2023 Jan 22;12(2):235. doi: 10.3390/antibiotics12020235.
The aim of the study is to evaluate the effect of the addition of selected fruits and herbs belonging to the "superfoods" category for the bioactivity of a rapeseed honey matrix. Flavored creamed honeys with nine types of various additives (2 and 4% of content) were prepared and analyzed for the content of total phenols, flavonoids, antioxidant (FRAP, DPPH and ABTS) and antibacterial activity against four strains of bacteria. Additionally, the impact of three months of storage on the antioxidant properties of the products obtained was examined. The significant dose-dependent increase in the content of bioactive ingredients and antioxidant capacity in spiced honeys, as compared to control honey, was observed. The highest enrichment was obtained for the addition of powdered sea buckthorn leaves and black raspberry fruits. Honey with the addition of sea buckthorn leaves inhibited the growth of , and whereas honeys with black raspberry and blackcurrant fruits showed activity only on the latter two strains. Furthermore, what is more interesting, honey supplemented with sea buckthorn leaf and black raspberry fruits inhibited biofilm formation at the sub-minimum inhibitory concentrations (sub-MICs), showing a dose-dependent anti-biofilm effect.
本研究的目的是评估添加属于“超级食物”类别的特定水果和草药对油菜籽蜂蜜基质生物活性的影响。制备了含有九种不同添加剂（含量分别为2%和4%）的调味奶油蜂蜜，并分析了其总酚、黄酮类化合物的含量、抗氧化能力（FRAP、DPPH和ABTS）以及对四种细菌菌株的抗菌活性。此外，还研究了三个月的储存对所得产品抗氧化性能的影响。与对照蜂蜜相比，观察到加香蜂蜜中生物活性成分含量和抗氧化能力显著呈剂量依赖性增加。添加沙棘叶粉和黑树莓果实的蜂蜜富集程度最高。添加沙棘叶的蜂蜜抑制了金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的生长，而添加黑树莓和黑加仑果实的蜂蜜仅对后两种菌株有活性。此外，更有趣的是，添加沙棘叶和黑树莓果实的蜂蜜在亚最低抑菌浓度（sub-MICs）下抑制了铜绿假单胞菌生物膜的形成，呈现出剂量依赖性的抗生物膜作用。
