Ramírez-Bayard I E, Mejía F, Medina-Sánchez J R, Cornejo-Reyes H, Castillo M, Querol-Audi J, Martínez-Torres A O
Experimental and Applied Microbiology Laboratory, Vice Rectory of Research and Postgraduate Affairs, Universidad de Panamá, Panama City 0820, Panama.
Water Microbiology Laboratory, Vice Rectory of Research and Postgraduate Affairs, Universidad de Panamá, Panama City 0820, Panama.
Antibiotics (Basel). 2023 Jan 31;12(2):280. doi: 10.3390/antibiotics12020280.
Antimicrobial resistance bacteria are nowadays ubiquitous. Its presence has been reported in almost every type of source, from water for agricultural and recreative use, water distribution pipes, and wastewater, to food, fomites, and clinical samples. Enterobacteriaceae, especially , are not the exception, showing an increased resistance to several antibiotics, causing a global health and economic burden. Therefore, the monitoring of fecal microbiota is important because it is present in numerous reservoirs where gene transfer between commensal and virulent bacteria can take place, representing a potential source of resistant . In this work, antibiotic resistance profiles of 150 isolates from environmental, animal, and human samples, collected in three rural areas in Panama, were analyzed. A total of 116 isolates were resistant to at least one of the nine antibiotics tested. Remarkably, almost 100% of these exhibited resistance to tetracycline. Plasmid-associated and genes were detected in 42.86% of the isolates analyzed, being the most prevalent. These results suggest that tetracycline resistance would be used as a convenient indicator of genetic horizontal transfer within a community.
如今，抗菌耐药菌无处不在。几乎在每一种来源中都有其存在的报道，从农业和娱乐用水、配水管道、废水，到食品、污染物和临床样本。特别是肠杆菌科也不例外，它们对多种抗生素的耐药性不断增加，造成了全球健康和经济负担。因此，粪便微生物群的监测很重要，因为它存在于许多宿主中，共生菌和致病细菌之间可能发生基因转移，这是耐药菌的一个潜在来源。在这项研究中，分析了从巴拿马三个农村地区采集的环境、动物和人类样本中的150株分离菌的抗生素耐药谱。共有116株分离菌对所测试的九种抗生素中的至少一种耐药。值得注意的是，其中几乎100%的分离菌对四环素耐药。在42.86%的分析分离菌中检测到了与质粒相关的耐药基因，其中tetM最为普遍。这些结果表明，四环素耐药性可作为一个群体内基因水平转移的便捷指标。
