Castro D J, Saxton R E, Fetterman H R, Castro D J, Ward P H
Division of Head and Neck Surgery (Otolaryngology), UCLA School of Medicine 90024.
Laryngoscope. 1987 Dec;97(12):1454-9. doi: 10.1288/00005537-198712000-00014.
Kodak Q-switch II is a new chemical with an absorption maxima at 1,051 nm, designed to be used as an Nd:YAG dye laser. The potential for this dye as a new chemosensitizing agent in the treatment of connective tissue diseases and wound healing with low energy Nd:YAG laser was examined. Two normal fibroblast cell lines were tested for sensitivity to various levels of this dye in vitro. These cells were exposed to Q-switch II dye at concentrations of 0.01, 0.1, 1, 10, 50, and 100 micrograms/ml for 1 and 24 hours. Cell viability was assessed by the trypan blue exclusion test. Cell duplication and DNA synthesis were measured by the incorporation of [3H]-thymidine at 6 and 24 hours postexposure to Q-switch II dye. At concentrations up to 10 micrograms/ml, both cell lines tested showed no changes in cell viability. However, at concentrations equal or higher than 50 micrograms/ml, more than 40% of the fibroblasts incorporated trypan blue after 24 hours of exposure to this dye, indicating significant cell destruction. The results indicate that Q-switch II dye is nontoxic to normal human fibroblast cultures and showed significant biostimulative effects on cell duplication at concentrations equal to or lower than 10 micrograms/ml. Further studies will be required to determine the usefulness of Q-switch II dye as a new photochemosensitizing agent for potential biostimulation of wound healing and/or treatment of connective tissue diseases with the Nd:YAG laser (near infrared, 1,060 nm) at "nonthermal" levels of energies.
柯达Q开关II是一种新型化学物质，其最大吸收波长为1051纳米，设计用作钕：钇铝石榴石染料激光器。研究了这种染料作为新型化学增敏剂在低能量钕：钇铝石榴石激光治疗结缔组织疾病和伤口愈合中的潜力。在体外测试了两种正常成纤维细胞系对不同浓度这种染料的敏感性。将这些细胞暴露于浓度为0.01、0.1、1、10、50和100微克/毫升的Q开关II染料中1小时和24小时。通过台盼蓝排斥试验评估细胞活力。在暴露于Q开关II染料后6小时和24小时，通过掺入[3H] - 胸腺嘧啶核苷来测量细胞增殖和DNA合成。在浓度高达10微克/毫升时，测试的两种细胞系的细胞活力均未发生变化。然而，在浓度等于或高于50微克/毫升时，在暴露于这种染料24小时后，超过40%的成纤维细胞摄取了台盼蓝，表明细胞受到显著破坏。结果表明，Q开关II染料对正常人成纤维细胞培养物无毒，并且在浓度等于或低于10微克/毫升时对细胞增殖显示出显著的生物刺激作用。需要进一步研究以确定Q开关II染料作为新型光化学增敏剂在“非热”能量水平下用钕：钇铝石榴石激光（近红外，1060纳米）潜在生物刺激伤口愈合和/或治疗结缔组织疾病中的实用性。
