Castro D J, Abergel R P, Meeker C, Dwyer R M, Lesavoy M A, Uitto J
Ann Plast Surg. 1983 Sep;11(3):214-22.
Human skin fibroblasts were subjected to treatment with a Neodymium:YAG laser at 1060 nm with varying levels of energy determined by a reproducible method of dosimetry. DNA synthesis in the cells was measured by the incorporation of [3H]thymidine, and collagen production was monitored by the synthesis of nondialyzable [3H]hydroxyproline after incubation of cells with [3H]proline. Using energy levels equal to 1.7 X 10(3) J/cm2, a significant reduction in DNA synthesis was noted, while the cells remained viable as tested by the trypan blue exclusion test. With energy levels higher or equal to 2.3 X 10(3) J/cm2, the suppression of DNA synthesis was accompanied by cell nonviability. The collagen production, when measured immediately following the treatment with 1.7 X 10(3) J/cm2, was markedly reduced, and similar effects were observed with higher energy levels. However, when the cells were tested for collagen production at 20 hours following laser treatment, there was a significant decrease in collagen production at energy levels as low as 1.1 X 10(3) J/cm2, a dose that did not affect DNA synthesis or cell viability. Thus, the results indicate that the Nd:YAG laser can selectively suppress collagen production without affecting cell proliferation. These observations suggest that laser treatment could potentially be used to reduce collagen deposition in conditions such as keloids and hypertrophic scars.
将人皮肤成纤维细胞用波长为1060nm的钕：钇铝石榴石激光进行处理，通过一种可重复的剂量测定方法确定不同的能量水平。细胞中的DNA合成通过[3H]胸腺嘧啶核苷的掺入来测量，并且在用[3H]脯氨酸孵育细胞后，通过不可透析的[3H]羟脯氨酸的合成来监测胶原蛋白的产生。使用等于1.7×10(3)J/cm2的能量水平时，注意到DNA合成显著减少，而通过台盼蓝排斥试验测试，细胞仍保持活力。当能量水平高于或等于2.3×10(3)J/cm2时，DNA合成的抑制伴随着细胞无活力。在用1.7×10(3)J/cm2处理后立即测量时，胶原蛋白的产生明显减少，在更高能量水平下也观察到类似的效果。然而，当在激光处理后20小时测试细胞的胶原蛋白产生时，在低至1.1×10(3)J/cm2的能量水平下胶原蛋白产生显著减少，该剂量不影响DNA合成或细胞活力。因此，结果表明钕：钇铝石榴石激光可以选择性地抑制胶原蛋白的产生而不影响细胞增殖。这些观察结果表明，激光治疗可能潜在地用于减少诸如瘢痕疙瘩和增生性瘢痕等病症中的胶原蛋白沉积。
