Deckelbaum L I, Lam J K, Cabin H S, Clubb K S, Long M B
Cardiology Section, West Haven Veterans Administration Medical Center, CT 06516.
Lasers Surg Med. 1987;7(4):330-5. doi: 10.1002/lsm.1900070406.
Precise targeting of laser energy to atherosclerotic plaque is crucial for the safe performance of laser angioplasty. The present study was designed to evaluate whether laser-induced fluorescence could distinguish atherosclerotic from normal aorta. Segments of human aorta obtained at necropsy were classified grossly and histologically as normal aorta (n = 7), thin yellow fatty plaque (n = 5), and thick white atheromatous plaque (n = 9), and analyzed by laser-induced fluorescence spectroscopy using a pulsed nitrogen laser. Fluorescence spectra were recorded over a wavelength range from 385 to 725 nm. Normal specimens had maximal fluorescence intensity at 514 nm. A prominent fluorescence peak at 448 nm was present in specimens characterized as white atheromatous plaque and at 538 nm in specimens characterized as yellow fatty plaque. The ratios of fluorescence intensity at 448 nm/514 nm and at 538 nm/514 nm correctly classified all specimens according to their gross and histologic type (p less than .001). Thus, a "smart" laser angioplasty catheter system might incorporate low-power laser radiation for arterial fluorescence spectroscopy to guide delivery of high-power laser radiation for plaque ablation.
将激光能量精确靶向至动脉粥样硬化斑块对于激光血管成形术的安全实施至关重要。本研究旨在评估激光诱导荧光是否能够区分动脉粥样硬化主动脉与正常主动脉。对尸检时获取的人主动脉节段进行大体和组织学分类，分为正常主动脉（n = 7）、薄黄色脂肪斑块（n = 5）和厚白色动脉粥样斑块（n = 9），并使用脉冲氮激光通过激光诱导荧光光谱法进行分析。在385至725 nm波长范围内记录荧光光谱。正常标本在514 nm处具有最大荧光强度。在被分类为白色动脉粥样斑块的标本中，448 nm处有一个明显的荧光峰，在被分类为黄色脂肪斑块的标本中，538 nm处有一个明显的荧光峰。448 nm/514 nm和538 nm/514 nm处的荧光强度比值根据其大体和组织学类型正确地对所有标本进行了分类（p小于0.001）。因此，一种“智能”激光血管成形术导管系统可能会结合低功率激光辐射用于动脉荧光光谱分析，以指导高功率激光辐射用于斑块消融的输送。
