和 基因的多种表达及其作为慢性稳定性心绞痛和急性冠状动脉综合征独立危险因素的潜在作用。
Various Expressions of and Genes and Their Potential Role as Independent Risk Factors for Chronic Stable Angina and Acute Coronary Syndrome.
机构信息
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Shebin el Kom 32511, Egypt.
Medical Biochemistry Unit, Department of Pathology, College of Medicine, Qassim University, Buraydah 51452, Saudi Arabia.
出版信息
Biomolecules. 2023 Feb 6;13(2):302. doi: 10.3390/biom13020302.
BACKGROUND AND AIM
Genetic factors play a significant role in the onset and progression of coronary artery disease (CAD). may contribute to the development of acute coronary syndrome (ACS) by affecting blood glucose levels and oxidative stress. The expression levels of were significantly higher in patients with unstable angina pectoris. However, the situation is different in ACS. In the current study, we aim to investigate the role of and as independent risk factors for chronic stable angina (CSA) and ACS.
SUBJECTS AND METHODS
This study involved 215 subjects (60 patients with CSA, 55 patients with ACS, and 100 controls). All subjects were exposed for assaying gene expressions of and by quantitative real-time polymerase chain reaction.
RESULTS
It was found that was upregulated, whereas was downregulated in patients with CAD compared to the control group. was significantly downregulated in patients with ACS compared to that in patients with CSA ( < 0.001), but was not ( = 0.7). was significantly upregulated in STEMI-ACS patients compared to CSA ( = 0.045) and NSTEMI ACS ( = 0.046), among non-diabetic ( = 0.023) smokers ( = 0.036) with hypertension ( = 0.005) and hypercholesterolemia ( = 0.001). ROC (receiver operating characteristic) curve analysis revealed that (0.981; < 0.001; 98.18) was the most sensitive mRNA for discriminating ACS from control, followed by (0.775; < 0.001; 70.91). However, for discriminating ACS from CSA combined mRNAs, () (0.893; < 0.001; 98.18) and (0.892; < 0.001; 81.82) are promising biomarkers. On the other hand, the most sensitive mRNA for differentiating CSA from control is mRNAs () (0.963; < 0.001; 95), then (81.3; < 0.001; 93.33), and finally, (0.782; < 0.001; 81.67). In the multivariate regression model, (( = 0.002), 0.118 (0.031-0.445)) and smoking status (( = 0.034); 0.151 (0.026-0.866)) were independent variables for ACS. Moreover, (( < 0.013); 0.706 (0.614-0.812)), Hb (( = 0.013); 0.525 (0.317-0.871)), and total cholesterol (( = 0.04); 0.865 (0.784-0.955)) were significantly ( < 0.05) and independently related to the prognosis of CSA. Furthermore, (( = 0.002), 0.923 (0.877-0.971)), (( = 0.001); 2.809 (1.558-5.064)) the body weight (( = 0.033); 1.254 (1.018-1.544)) were independently associated with CSA.
CONCLUSIONS
Our study concluded that the dysregulated mRNA and gene expressions may be useful in diagnosis of CAD and prediction of ACS development.
背景与目的
遗传因素在冠心病（CAD）的发病和进展中起重要作用。可能通过影响血糖水平和氧化应激来促进急性冠状动脉综合征（ACS）的发展。不稳定型心绞痛患者的表达水平显著升高。然而，ACS 的情况则不同。在目前的研究中，我们旨在研究和作为慢性稳定型心绞痛（CSA）和 ACS 的独立危险因素的作用。
对象与方法
本研究共纳入 215 例患者（60 例 CSA 患者，55 例 ACS 患者和 100 例对照）。所有患者均通过定量实时聚合酶链反应检测和的基因表达。
结果
与对照组相比，CAD 患者中上调，而下调。与 CSA 患者相比，ACS 患者显著下调（<0.001），但（=0.7）。与 CSA 相比，STEMI-ACS 患者（=0.045）和 NSTEMI ACS 患者（=0.046）中的非糖尿病（=0.023）吸烟者（=0.036）的高血压（=0.005）和高胆固醇血症（=0.001）患者中显著上调。ROC（接收者操作特征）曲线分析显示，（0.981；<0.001；98.18）是最敏感的 mRNA 用于区分 ACS 与对照，其次是（0.775；<0.001；70.91）。然而，对于区分 ACS 与 CSA 联合 mRNAs，（）（0.893；<0.001；98.18）和（0.892；<0.001；81.82）是有前途的生物标志物。另一方面，区分 CSA 与对照的最敏感的 mRNA 是（）（0.963；<0.001；95），其次是（81.3；<0.001；93.33），最后是（0.782；<0.001；81.67）。在多变量回归模型中，（=0.002），0.118（0.031-0.445））和吸烟状态（=0.034）；0.151（0.026-0.866））是 ACS 的独立变量。此外，（<0.013）；0.706（0.614-0.812）），Hb（=0.013）；0.525（0.317-0.871））和总胆固醇（=0.04）；0.865（0.784-0.955））显著（<0.05）和独立相关 CSA 的预后。此外，（=0.002），0.923（0.877-0.971）），（=0.001）；2.809（1.558-5.064））体重（=0.033）；1.254（1.018-1.544））与 CSA 独立相关。
结论
我们的研究表明，失调的 mRNA 和基因表达可能有助于 CAD 的诊断和 ACS 发展的预测。
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