Zhang Kejing, Mi Fang, Li Xuelan, Wang Zhiying, Jiang Fugui, Song Enliang, Guo Peng, Lan Xianyong
College of Animal Science and Technology, Northwest A&F University, Yangling, China.
National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Anim Biotechnol. 2023 Dec;34(8):3387-3394. doi: 10.1080/10495398.2022.2149547. Epub 2022 Nov 30.
The biological clock (also known as circadian clock) is closely related to growth and development, metabolism, and diseases in animals. As a part of the circadian clock, the cryptochrome circadian regulator 1 () gene is involved in the regulation of biological processes such as osteogenesis, energy metabolism and cell proliferation, however, few studies have been reported on the relationship between this gene and animal carcass traits. Herein, a total of four insertion/deletion (InDel) loci within the gene were detected in Shandong Black Cattle Genetic Resource (SDBCGR) population ( = 433). Among them, the P1-6-bp-del locus was polymorphic in population of interest. Moreover, the P1-6-bp-del locus showed two genotypes, with a higher insertion/insertion (II) genotype frequency (0.751) than insertion/deletion (ID) genotype frequency (0.249). Correlation analysis showed that the P1-6-bp-del locus polymorphisms were significantly associated with twenty carcass traits (e.g., slaughter weight, limb weight, and belly meat weight). Individuals with II genotype were significantly better than those with ID genotype for eighteen carcass traits. Therefore, the P1-6-bp-del locus of the gene can be used as a molecular marker for beef cattle breeding.
生物钟（也称为昼夜节律钟）与动物的生长发育、新陈代谢及疾病密切相关。作为昼夜节律钟的一部分，隐花色素昼夜调节因子1（）基因参与骨生成、能量代谢和细胞增殖等生物学过程的调控，然而，关于该基因与动物胴体性状之间的关系鲜有研究报道。在此，在山东黑牛遗传资源（SDBCGR）群体（n = 433）中检测到该基因内共4个插入/缺失（InDel）位点。其中，P1-6-bp-del位点在目标群体中具有多态性。此外，P1-6-bp-del位点表现出两种基因型，插入/插入（II）基因型频率（0.751）高于插入/缺失（ID）基因型频率（0.249）。相关性分析表明，P1-6-bp-del位点多态性与20个胴体性状（如屠宰体重、四肢重量和腹肉重量）显著相关。II基因型个体在18个胴体性状上显著优于ID基因型个体。因此，该基因的P1-6-bp-del位点可作为肉牛育种的分子标记。
