Li Xuelan, Jiang Enhui, Zhang Kejing, Zhang Sihuan, Jiang Fugui, Song Enliang, Chen Hong, Guo Peng, Lan Xianyong
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China.
Institute of Animal Science and Veterinary, Shandong Academy of Agriculture Science, Jinan 250100, China.
Animals (Basel). 2022 Jun 23;12(13):1616. doi: 10.3390/ani12131616.
As an important part of the circadian rhythm, the circadian regulation factor 2 of cryptochrome (CRY2), regulates many physiological functions. Previous studies have reported that CRY2 is involved in growth and development. However, the relationship between CRY2 gene polymorphism and cattle carcass traits remains unclear. The aim of this study was to detect the possible variations of the CRY2 gene and elucidate the association between the CRY2 gene and carcass traits in the Shandong Black Cattle Genetic Resource (SDBCGR) population (n = 705). We identified a 24-bp deletion variation (CRY2-P6) and a 6-bp insertion variation (CRY2-P7) in the bovine CRY2 gene. The frequency of the homozygous II genotype is higher than the heterozygous ID genotype in both two loci. In addition, CRY2-P6 was consistent with HWE (p > 0.05). Importantly, the CRY2-P6 variant was significantly associated with 12 carcass traits, including gross weight, ribeye, high rib, thick flank, etc. and the II was the dominant genotype. The CRY2-P7 site was also significantly correlated with five traits (gross weight, beef-tongue, etc.). Collectively, these outcomes indicated that the two Indel loci in the CRY2 gene could be used for marker-assisted selection of cattle carcass traits.
作为昼夜节律的重要组成部分，隐花色素2（CRY2）的昼夜调节因子调控着许多生理功能。以往研究报道CRY2参与生长发育。然而，CRY2基因多态性与牛胴体性状之间的关系尚不清楚。本研究旨在检测山东黑牛遗传资源群体（SDBCGR，n = 705）中CRY2基因的可能变异，并阐明CRY2基因与胴体性状之间的关联。我们在牛CRY2基因中鉴定出一个24bp的缺失变异（CRY2-P6）和一个6bp的插入变异（CRY2-P7）。在这两个位点，纯合II基因型的频率均高于杂合ID基因型。此外，CRY2-P6符合哈迪-温伯格平衡（p > 0.05）。重要的是，CRY-2P6变异与包括毛重、眼肌面积、上腰部、后腰肉厚度等12个胴体性状显著相关，且II型是优势基因型。CRY2-P7位点也与5个性状（毛重、牛舌等）显著相关。总体而言，这些结果表明CRY2基因中的两个插入缺失位点可用于牛胴体性状的标记辅助选择。
