• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

逆转录病毒介导的人腺苷脱氨酶基因转移：功能性酶在小鼠体内造血干细胞中的表达。

Retrovirus-mediated gene transfer of human adenosine deaminase: expression of functional enzyme in murine hematopoietic stem cells in vivo.

作者信息

Lim B, Williams D A, Orkin S H

机构信息

Division of Hematology-Oncology, Children's Hospital, Boston, Massachusetts.

出版信息

Mol Cell Biol. 1987 Oct;7(10):3459-65. doi: 10.1128/mcb.7.10.3459-3465.1987.

DOI:10.1128/mcb.7.10.3459-3465.1987
PMID:3683389
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367997/
Abstract

Simplified Moloney murine leukemia virus-based recombinant retrovirus vectors have been constructed which transduce human adenosine deaminase (ADA) cDNA. ADA transcription is under the control of the constitutive promoter for the human X chromosome phosphoglycerate kinase (pgk) gene. In these simplified vectors, dominant selectable markers are not included and selection is dependent on overproduction of functional ADA enzyme. Primary murine hematopoietic cells were infected with helper-free recombinant ADA virus generated from Psi-2 packaging cells. Protein analysis revealed that human ADA enzyme was expressed in progenitor-derived hematopoietic colonies in vitro and CFU-S-derived spleen colonies in vivo. Enzyme expression was dependent on transcription from the pgk promoter. ADA expression in primary murine hematopoietic cells directed by the internal promoter was not adversely affected by the presence of the Moloney virus long terminal repeat enhancer sequence. Use of these vectors allows systematic evaluation of the effects of specific sequences in recombinant retrovirus vectors on expression in primary murine hematopoietic cells in vivo.

摘要

已构建出基于简化型莫洛尼鼠白血病病毒的重组逆转录病毒载体，其可转导人腺苷脱氨酶（ADA）cDNA。ADA转录受人类X染色体磷酸甘油酸激酶（pgk）基因组成型启动子的控制。在这些简化载体中，不包含显性选择标记，选择依赖于功能性ADA酶的过量产生。用来自Psi-2包装细胞产生的无辅助重组ADA病毒感染原代鼠造血细胞。蛋白质分析显示，人ADA酶在体外祖细胞来源的造血集落以及体内CFU-S来源的脾集落中表达。酶表达依赖于pgk启动子的转录。由内部启动子指导的原代鼠造血细胞中的ADA表达不受莫洛尼病毒长末端重复增强子序列存在的不利影响。使用这些载体能够系统评估重组逆转录病毒载体中特定序列对体内原代鼠造血细胞表达的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72af/367997/172a69b4dbaf/molcellb00082-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72af/367997/83a2f3091f54/molcellb00082-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72af/367997/e5ec629576b5/molcellb00082-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72af/367997/b927fe0b1b20/molcellb00082-0102-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72af/367997/172a69b4dbaf/molcellb00082-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72af/367997/83a2f3091f54/molcellb00082-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72af/367997/e5ec629576b5/molcellb00082-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72af/367997/b927fe0b1b20/molcellb00082-0102-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72af/367997/172a69b4dbaf/molcellb00082-0103-a.jpg

相似文献

1
Retrovirus-mediated gene transfer of human adenosine deaminase: expression of functional enzyme in murine hematopoietic stem cells in vivo.逆转录病毒介导的人腺苷脱氨酶基因转移：功能性酶在小鼠体内造血干细胞中的表达。
Mol Cell Biol. 1987 Oct;7(10):3459-65. doi: 10.1128/mcb.7.10.3459-3465.1987.
2
Retrovirus-mediated transfer of human adenosine deaminase gene sequences into cells in culture and into murine hematopoietic cells in vivo.逆转录病毒介导的人腺苷脱氨酶基因序列向培养细胞及体内小鼠造血细胞的转移。
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2566-70. doi: 10.1073/pnas.83.8.2566.
3
Long-term expression of human adenosine deaminase in mice transplanted with retrovirus-infected hematopoietic stem cells.人腺苷脱氨酶在移植了逆转录病毒感染的造血干细胞的小鼠中的长期表达。
Proc Natl Acad Sci U S A. 1989 Nov;86(22):8892-6. doi: 10.1073/pnas.86.22.8892.
4
Retroviral-mediated gene transfer into mammalian cells.逆转录病毒介导的基因转移至哺乳动物细胞
Blood Cells. 1987;13(1-2):285-98.
5
Expression of human adenosine deaminase from various strong promoters after gene transfer into human hematopoietic cell lines.基因转入人造血细胞系后，不同强启动子驱动的人腺苷脱氨酶的表达
Blood. 1989 Aug 1;74(2):876-81.
6
Transfer and expression of human ADA in murine hematopoietic stem cells.人腺苷脱氨酶在小鼠造血干细胞中的转移与表达
Prog Clin Biol Res. 1987;251:567-80.
7
Human purine nucleoside phosphorylase and adenosine deaminase: gene transfer into cultured cells and murine hematopoietic stem cells by using recombinant amphotropic retroviruses.人嘌呤核苷磷酸化酶和腺苷脱氨酶：利用重组双嗜性逆转录病毒将基因导入培养细胞和小鼠造血干细胞
Mol Cell Biol. 1987 Feb;7(2):838-46. doi: 10.1128/mcb.7.2.838-846.1987.
8
Expression of human adenosine deaminase in mice transplanted with hemopoietic stem cells infected with amphotropic retroviruses.人腺苷脱氨酶在移植了感染嗜双嗜性逆转录病毒的造血干细胞的小鼠中的表达。
J Exp Med. 1990 Sep 1;172(3):729-36. doi: 10.1084/jem.172.3.729.
9
Expression of human adenosine deaminase in murine hematopoietic cells.人腺苷脱氨酶在小鼠造血细胞中的表达。
Mol Cell Biol. 1988 Dec;8(12):5116-25. doi: 10.1128/mcb.8.12.5116-5125.1988.
10
Restriction of expression of an integrated recombinant retrovirus in primary but not immortalized murine hematopoietic stem cells.整合的重组逆转录病毒在原代而非永生化的小鼠造血干细胞中的表达受限。
Blood. 1988 Jun;71(6):1738-43.

引用本文的文献

1
Elements of lentiviral vector design toward gene therapy for treating mucopolysaccharidosis I.用于治疗黏多糖贮积症I型基因治疗的慢病毒载体设计要素。
Mol Genet Metab Rep. 2016 Aug 13;8:87-93. doi: 10.1016/j.ymgmr.2015.11.004. eCollection 2016 Sep.
2
High-efficiency gene transfer into normal and adenosine deaminase-deficient T lymphocytes is mediated by transduction on recombinant fibronectin fragments.通过重组纤连蛋白片段转导介导，可实现向正常及腺苷脱氨酶缺陷型T淋巴细胞的高效基因转移。
J Virol. 1998 Jun;72(6):4882-92. doi: 10.1128/JVI.72.6.4882-4892.1998.
3
Systemic T cell-independent tumor immunity after transplantation of universal receptor-modified bone marrow into SCID mice.

本文引用的文献

1
A direct measurement of the radiation sensitivity of normal mouse bone marrow cells.正常小鼠骨髓细胞辐射敏感性的直接测量。
Radiat Res. 1961 Feb;14:213-22.
2
De novo methylation, expression, and infectivity of retroviral genomes introduced into embryonal carcinoma cells.导入胚胎癌细胞的逆转录病毒基因组的从头甲基化、表达及感染性。
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4098-102. doi: 10.1073/pnas.79.13.4098.
3
Self-renewal of hemopoietic stem cells during mixed colony formation in vitro.体外混合集落形成过程中造血干细胞的自我更新
将通用受体修饰的骨髓移植到SCID小鼠体内后的全身性非T细胞依赖性肿瘤免疫。
J Exp Med. 1996 Dec 1;184(6):2261-9. doi: 10.1084/jem.184.6.2261.
4
Self-contained, tetracycline-regulated retroviral vector system for gene delivery to mammalian cells.用于将基因递送至哺乳动物细胞的独立四环素调控逆转录病毒载体系统。
J Virol. 1996 Jan;70(1):62-7. doi: 10.1128/JVI.70.1.62-67.1996.
5
Human cord blood cells as targets for gene transfer: potential use in genetic therapies of severe combined immunodeficiency disease.人脐带血细胞作为基因转移的靶标：在重症联合免疫缺陷病基因治疗中的潜在应用。
J Exp Med. 1993 Aug 1;178(2):529-36. doi: 10.1084/jem.178.2.529.
6
High-efficiency identification of genes by functional analysis from a retroviral cDNA expression library.通过对逆转录病毒cDNA表达文库进行功能分析高效鉴定基因
J Virol. 1994 Sep;68(9):5523-31. doi: 10.1128/JVI.68.9.5523-5531.1994.
7
Retrovirus-mediated transfer of MHC class II cDNA into swine bone marrow cells.逆转录病毒介导的MHC II类cDNA转入猪骨髓细胞
J Mol Med (Berl). 1995 Jun;73(6):269-78. doi: 10.1007/BF00231613.
8
Expression of the human beta-globin gene following retroviral-mediated transfer into multipotential hematopoietic progenitors of mice.逆转录病毒介导的人β-珠蛋白基因转移至小鼠多能造血祖细胞后的表达
Proc Natl Acad Sci U S A. 1988 Aug;85(16):6062-6. doi: 10.1073/pnas.85.16.6062.
9
Comparison of expression in hemopoietic cells by retroviral vectors carrying two genes.携带两个基因的逆转录病毒载体在造血细胞中的表达比较。
J Virol. 1988 Jul;62(7):2464-73. doi: 10.1128/JVI.62.7.2464-2473.1988.
10
Retrovirus mediated gene transfer into hemopoietic cells.
Blut. 1988 Jul;57(1):1-5. doi: 10.1007/BF00320626.
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3629-33. doi: 10.1073/pnas.78.6.3629.
4
Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.逆转录病毒包装突变体的构建及其用于产生无辅助病毒的缺陷型逆转录病毒。
Cell. 1983 May;33(1):153-9. doi: 10.1016/0092-8674(83)90344-6.
5
Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase.人X染色体编码的磷酸甘油酸激酶全长cDNA克隆的分离与DNA序列分析
Proc Natl Acad Sci U S A. 1983 Jan;80(2):472-6. doi: 10.1073/pnas.80.2.472.
6
Infectious and selectable retrovirus containing an inducible rat growth hormone minigene.含有可诱导大鼠生长激素小基因的传染性和可选择逆转录病毒。
Science. 1984 Sep 7;225(4666):993-8. doi: 10.1126/science.6089340.
7
Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse.将新的遗传物质导入小鼠的多能造血干细胞中。
Nature. 1984;310(5977):476-80. doi: 10.1038/310476a0.
8
Erythroid colony formation in cultures of mouse and human bone marrow: analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose-concanavalin A.小鼠和人骨髓培养中的红系集落形成：通过琼脂糖-伴刀豆球蛋白A凝胶过滤和亲和层析分析对促红细胞生成素的需求
J Cell Physiol. 1974 Apr;83(2):309-20. doi: 10.1002/jcp.1040830218.
9
The growth of mouse bone marrow cells in vitro.小鼠骨髓细胞的体外生长
Aust J Exp Biol Med Sci. 1966 Jun;44(3):287-99. doi: 10.1038/icb.1966.28.
10
Introduction of a selectable gene into primitive stem cells capable of long-term reconstitution of the hemopoietic system of W/Wv mice.将一个可选择基因导入能够长期重建W/Wv小鼠造血系统的原始干细胞中。
Cell. 1985 Aug;42(1):71-9. doi: 10.1016/s0092-8674(85)80102-1.