用于实验性切口伤口细菌定量的湿拭子法与组织活检法的比较。

Comparison between moist swab and tissue biopsy methods for quantitation of bacteria in experimental incisional wounds.

作者信息

Bornside G H, Bornside B B

出版信息

J Trauma. 1979 Feb;19(2):103-5. doi: 10.1097/00005373-197902000-00005.

DOI:10.1097/00005373-197902000-00005

Abstract

The relationship between tissue-associated and wound fluid-associated levels of bacteria in closed, healing experimental incisional wounds seeded with Escherichia coli was examined in 200 rats. Tissue specimens and moist swab specimens were taken simultaneously and cultured by a single plate serial dilution method. Colony counts of approximately 10(5) bacteria/gm of tissue were found to be equivalent to colony counts of 10(3) bacteria/ml of specimen obtained on a moist swab. Moist swab sampling of an incisional wound therefore offers a direct and simple method of ascertaining infection (defined by others as greater than 10(5) bacteria/gm of tissue). Moist swab sampling obviates the necessity of surgical manipulation of the patient and subsequent weighing and grinding of tissue in the laboratory.

摘要

在200只接种大肠杆菌的闭合性愈合实验性切口伤口的大鼠中，研究了组织相关细菌水平与伤口液相关细菌水平之间的关系。同时采集组织标本和湿拭子标本，并通过单平板连续稀释法进行培养。发现每克组织中约10⁵个细菌的菌落计数等同于在湿拭子上获得的每毫升标本中10³个细菌的菌落计数。因此，对切口伤口进行湿拭子采样提供了一种直接且简单的确定感染的方法（其他人定义为每克组织中细菌数大于10⁵）。湿拭子采样避免了对患者进行手术操作以及随后在实验室中对组织进行称重和研磨的必要性。