Jung Ju-Young, Min Cheol Woo, Jang Jeong Woo, Gupta Ravi, Kim Ji-Hyun, Kim Young-Hun, Cho Sung Won, Song Young Hun, Jo Ick-Hyun, Rakwal Randeep, Kim Yu-Jin, Kim Sun Tae
Department of Plant Bioscience, Pusan National University, Miryang 50463, Republic of Korea.
College of General Education, Kookmin University, Seoul 02707, Republic of Korea.
Int J Mol Sci. 2023 Feb 12;24(4):3693. doi: 10.3390/ijms24043693.
Ginseng, an important crop in East Asia, exhibits multiple medicinal and nutritional benefits because of the presence of ginsenosides. On the other hand, the ginseng yield is severely affected by abiotic stressors, particularly salinity, which reduces yield and quality. Therefore, efforts are needed to improve the ginseng yield during salinity stress, but salinity stress-induced changes in ginseng are poorly understood, particularly at the proteome-wide level. In this study, we report the comparative proteome profiles of ginseng leaves at four different time points (mock, 24, 72, and 96 h) using a label-free quantitative proteome approach. Of the 2484 proteins identified, 468 were salt-responsive. In particular, glycosyl hydrolase 17 (), catalase-peroxidase 2, voltage-gated potassium channel subunit beta-2, fructose-1,6-bisphosphatase class 1, and chlorophyll a-b binding protein accumulated in ginseng leaves in response to salt stress. The heterologous expression of in improved the salt tolerance of transgenic lines without compromising plant growth. Overall, this study uncovers the salt-induced changes in ginseng leaves at the proteome level and highlights the critical role of in salt stress tolerance in ginseng.
人参是东亚一种重要的作物，由于含有人参皂苷，具有多种药用和营养益处。另一方面，人参产量受到非生物胁迫因素的严重影响，尤其是盐度，这会降低产量和品质。因此，需要努力提高盐胁迫期间的人参产量，但人参中盐胁迫诱导的变化了解甚少，尤其是在全蛋白质组水平上。在本研究中，我们使用无标记定量蛋白质组方法报告了人参叶片在四个不同时间点（模拟、24、72和96小时）的比较蛋白质组图谱。在鉴定出的2484种蛋白质中，有468种是盐响应性的。特别是，糖基水解酶17、过氧化氢酶-过氧化物酶2、电压门控钾通道亚基β-2、1,6-二磷酸果糖磷酸酶1类和叶绿素a-b结合蛋白在人参叶片中因盐胁迫而积累。在拟南芥中异源表达该蛋白提高了转基因株系的耐盐性，且不影响植物生长。总体而言，本研究揭示了蛋白质组水平上盐胁迫诱导的人参叶片变化，并突出了该蛋白在人参耐盐胁迫中的关键作用。
