Kong Wei-Liang, Ni Hang, Wang Wei-Yu, Wu Xiao-Qin
Co-innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, China.
Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, China.
Front Microbiol. 2022 Sep 21;13:1013468. doi: 10.3389/fmicb.2022.1013468. eCollection 2022.
Volatile organic compounds (VOCs) produced by microorganisms are considered promising environmental-safety fumigants for controlling soil-borne diseases. , a notorious fungal pathogen, causes economically important wilt diseases in agriculture and forestry industries. Here, we determined the antifungal activity of VOCs produced by T2. The VOCs from T2 were trapped by solid-phase microextraction (SPME) and tentatively identified through gas chromatography-mass spectrometry (GC/MS). The microsclerotia formation, cell wall-degrading enzymes and melanin synthesis of exposed to the VOC mixtures and selected single standards were examined. The results showed that the VOCs produced by strain T2 significantly inhibited the growth of mycelium and reduced the severity of wilt in tobacco and cotton. Six individual compounds were identified in the volatilome of T2, and the dominant compounds were 3-octanone, 3-methyl-1-butanol, butanoic acid ethyl ester and 2-hexyl-furan. The VOCs of strain T2 exert a significant inhibitory effect on microsclerotia formation and decreased the activities of pectin lyase and endo-β-1,4-glucanase in . VOCs also downregulated the , , and genes related to melanin synthesis by 29. 41-, 10. 49-, and 3.11-fold, respectively. Therefore, T2 has potential as a promising biofumigant for the biocontrol of wilt disease.
微生物产生的挥发性有机化合物（VOCs）被认为是控制土传病害的有前景的环境安全熏蒸剂。，一种臭名昭著的真菌病原体，在农业和林业中引发具有重要经济影响的枯萎病。在此，我们测定了T2产生的VOCs的抗真菌活性。T2产生的VOCs通过固相微萃取（SPME）捕集，并通过气相色谱 - 质谱联用（GC/MS）进行初步鉴定。检测了暴露于VOC混合物和选定单一标准品的的微菌核形成、细胞壁降解酶和黑色素合成。结果表明，菌株T2产生的VOCs显著抑制了的菌丝体生长，并降低了烟草和棉花枯萎病的严重程度。在T2的挥发物组中鉴定出6种单一化合物，主要化合物为3 - 辛酮、3 - 甲基 - 1 - 丁醇、丁酸乙酯和2 - 己基呋喃。菌株T2的VOCs对微菌核形成具有显著抑制作用，并降低了中的果胶裂解酶和内切β - 1,4 - 葡聚糖酶的活性。VOCs还分别下调了与黑色素合成相关的、和基因29.41倍、10.49倍和3.11倍。因此，T2有潜力作为一种有前景的生物熏蒸剂用于枯萎病的生物防治。
