Mufti Afoua, Contreras María Del Mar, Gómez-Cruz Irene, Alshamrani Abdullah, Nahdi Saber, Mansour Lamjed, Alwasel Salah, Harrath Abdel Halim, Tlili Nizar
Laboratory of Biotechnology and Biomonitoring of the Environment and Oasis Ecosystems, Faculty of Sciences of Gafsa, Gafsa 2112, Tunisia.
Department of Chemical, Environmental and Materials Engineering, Centre for Advanced Studies in Earth Sciences, Energy and Environment (CEACTEMA), Universidad de Jaén, Campus Las Lagunillas, 23071 Jaén, Spain.
Life (Basel). 2023 Jan 23;13(2):323. doi: 10.3390/life13020323.
The aim of the present study was to examine, for the first time, the phytochemical content of pulp extract (EAP) and explore its antioxidant and anti-inflammatory capacities. High-performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometry (HPLC-ESI-QTOF/MS) was used for phytochemical analysis and three in vitro antioxidant assays together with three in vitro anti-inflammatory tests were used for the assessment of biological activity. The HPLC-ESI-QTOF/MS analysis revealed the presence of 42 metabolites, including flavonoids, sphingolipides, fatty acids, ephedrine derivatives, and amino acid derivatives. In vitro findings revealed that EAP has interesting 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide, and ferrous ion chelating capacities (IC values were 0.57 mg/mL, 0.55 mg/mL, and 0.51 mg/mL for DPPH, superoxide radical, and ferrous ion, respectively). Furthermore, EAP showed a noticeable anti-inflammatory ability by inhibiting the two cyclooxygenase isoforms, COX-1 and COX-2 (IC of 59.1 and 58.8 µg/mL for COX-1 and COX-2, respectively), preventing protein denaturation (IC50 = 0.51 mg/mL), and protecting membrane stabilization (IC50 = 0.53 mg/mL). The results highlighted the use of pulp as a potential source of natural compounds with therapeutic effects for the management of inflammatory disorders.
本研究的目的是首次检测果肉提取物（EAP）的植物化学成分，并探索其抗氧化和抗炎能力。采用高效液相色谱-电喷雾电离-四极杆-飞行时间质谱（HPLC-ESI-QTOF/MS）进行植物化学成分分析，并使用三种体外抗氧化试验和三种体外抗炎试验来评估其生物活性。HPLC-ESI-QTOF/MS分析显示存在42种代谢物，包括黄酮类化合物、鞘脂、脂肪酸、麻黄碱衍生物和氨基酸衍生物。体外研究结果表明，EAP具有有趣的2,2-二苯基-1-苦基肼（DPPH）、超氧化物和亚铁离子螯合能力（DPPH、超氧自由基和亚铁离子的IC值分别为0.57 mg/mL、0.55 mg/mL和0.51 mg/mL）。此外，EAP通过抑制两种环氧化酶同工型COX-1和COX-2（COX-1和COX-2的IC分别为59.1和58.8 µg/mL）、防止蛋白质变性（IC50 = 0.51 mg/mL）和保护膜稳定性（IC50 = 0.53 mg/mL）显示出显著的抗炎能力。结果强调了果肉作为具有治疗作用的天然化合物潜在来源在炎症性疾病管理中的应用。
