Pahalagedara Amila S N W, Flint Steve, Palmer Jon, Brightwell Gale, Luo Xian, Li Liang, Gupta Tanushree B
Food System Integrity Team, AgResearch Ltd., Hopkirk Research Institute, Massey University, Palmerston North 4474, New Zealand.
School of Food and Advanced Technology, Massey University, Palmerston North 4442, New Zealand.
Metabolites. 2023 Feb 9;13(2):252. doi: 10.3390/metabo13020252.
This work focused on the metabolomic profiling of the conditioned medium (FS03CM) produced by an anaerobic bacterium closely related to spp. to identify potential antimicrobial metabolites. The metabolome of the conditioned medium was profiled by two-channel Chemical Isotope Labelling (CIL) LC-MS. The detected metabolites were identified or matched by conducting a library search using different confidence levels. Forty-eight significantly changed metabolites were identified with high confidence after the growth of isolate FS03 in cooked meat glucose starch (CMGS) medium. Some of the secondary metabolites identified with known antimicrobial activities were 4-hydroxyphenyllactate, 3-hydroxyphenylacetic acid, acetic acid, isobutyric acid, valeric acid, and tryptamine. Our findings revealed the presence of different secondary metabolites with previously reported antimicrobial activities and suggested the capability of producing antimicrobial metabolites by the anaerobic bacterium FS03.
这项工作聚焦于一种与[具体菌种名称]密切相关的厌氧菌所产生的条件培养基（FS03CM）的代谢组学分析，以鉴定潜在的抗菌代谢物。通过双通道化学同位素标记（CIL）液相色谱 - 质谱联用技术对条件培养基的代谢组进行分析。通过使用不同置信水平进行库搜索来鉴定或匹配检测到的代谢物。在分离株FS03于熟肉葡萄糖淀粉（CMGS）培养基中生长后，高置信度地鉴定出48种显著变化的代谢物。一些已鉴定出具有已知抗菌活性的次生代谢物包括4 - 羟基苯乳酸、3 - 羟基苯乙酸、乙酸、异丁酸、戊酸和色胺。我们的研究结果揭示了存在具有先前报道的抗菌活性的不同次生代谢物，并表明厌氧菌FS03具有产生抗菌代谢物的能力。
