Fougère Laëtitia, Zubrzycki Sandrine, Elfakir Claire, Destandau Emilie
Institut de Chimie Organique et Analytique, Université d'Orléans, CNRS, UMR 7311, F-45067 Orléans, France.
Plants (Basel). 2023 Feb 6;12(4):721. doi: 10.3390/plants12040721.
In addition to having different biological activities of interest, corn silks play a role in the defense of plants. While benzoxamines and flavonoids have already been identified as molecules of plant defense and growth mechanisms, knowledge on the phytochemical composition of corn silk is lacking. Such knowledge would make it possible to better select the most effective varieties to improve resistance or bioactive properties. In this article, an approach was implemented to map a corn silk extract in two complementary ways. The first one involved working with UHPLC/HRMS data and Kendrick and van Krevelen plots to highlight a homologous series of compounds, such as lipids from 17 to 23 carbons, monoglycosylated flavonoids from 21 to 24 carbons, diglycosylated flavonoids of 26 to 28 carbons and organic acids of 14 to 19 carbons. The second way was to analyze the sample in UHPLC/HRMS and to plot mass spectral similarity networks with the GNPS platform and Cytoscape software to refine identification. By combining the information obtained, we were able to propose an identification for 104 detected molecules, including 7 nitrogenous, 28 lipidic and 67 phenolic compounds, leading to the first detailed phytochemical analysis of corn silk extract.
除了具有不同的生物学活性外，玉米须在植物防御中也发挥着作用。虽然苯并恶唑嗪酮和黄酮类化合物已被确定为植物防御和生长机制的分子，但对玉米须的植物化学成分缺乏了解。这些知识将有助于更好地选择最有效的品种，以提高抗性或生物活性。在本文中，采用了一种方法以两种互补方式绘制玉米须提取物图谱。第一种方法是处理超高效液相色谱/高分辨率质谱（UHPLC/HRMS）数据以及肯德里克（Kendrick）和范克雷维伦（van Krevelen）图，以突出一系列同源化合物，例如含17至23个碳的脂质、含21至24个碳的单糖基化黄酮类化合物、含26至28个碳的双糖基化黄酮类化合物以及含14至19个碳的有机酸。第二种方法是在UHPLC/HRMS中分析样品，并使用全球天然产物社会分子网络（GNPS）平台和Cytoscape软件绘制质谱相似性网络以完善鉴定。通过结合所获得的信息，我们能够对104种检测到的分子提出鉴定结果，包括7种含氮化合物、28种脂质化合物和67种酚类化合物，从而首次对玉米须提取物进行了详细的植物化学分析。
