Netshimbupfe Mmbulaheni Happiness, Berner Jacques, Van Der Kooy Frank, Oladimeji Olakunle, Gouws Chrisna
Unit for Environmental Science and Management, North-West University (Potchefstroom Campus), Potchefstroom 2520, South Africa.
Centre of Excellence for Pharmaceutical Sciences (Pharmacen™), North-West University, Potchefstroom 2520, South Africa.
Plants (Basel). 2023 Feb 20;12(4):953. doi: 10.3390/plants12040953.
Drought and heat stress is known to influence the accumulation of mineral content, antioxidant activity, phenolics, flavonoids and other bioactive compounds in many tolerant leafy vegetables. plants can tolerate adverse weather conditions, especially drought and heat. Therefore, evaluating the influence of drought and heat stress on commercially and medically important crop species like is important to grow the crop for optimal nutritional and medicinal properties. This study investigated the influence of drought and heat stress and a combination of both on the accumulation of phenolic and flavonoid compounds and the antioxidant capacity of African , , and . Phenolic and flavonoid compounds were extracted with methanol and aqueous solvents and were quantified using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Caffeic acid was the main phenolic compound identified in aqueous extracts of and . Rutin was the most abundant flavonoid compound in all the species tested, with the highest concentration found in . The results suggest a strong positive, but species and compound-specific effect of drought and heat stress on bioactive compounds accumulation. We concluded that heat stress at 40 °C under well-watered conditions and combined drought and heat stress (at 30 °C and 35 °C) appeared to induce the accumulation of caffeic acid and rutin. Hence, cultivation of these species in semi-arid and arid areas is feasible.
已知干旱和热胁迫会影响许多耐受性叶菜类蔬菜中矿物质含量、抗氧化活性、酚类、黄酮类和其他生物活性化合物的积累。植物能够耐受恶劣的天气条件，尤其是干旱和高温。因此，评估干旱和热胁迫对像[具体作物名称未给出]这样具有商业和医学重要性的作物品种的影响，对于培育具有最佳营养和药用特性的作物至关重要。本研究调查了干旱、热胁迫以及二者组合对非洲[具体作物名称未给出]、[具体作物名称未给出]、[具体作物名称未给出]和[具体作物名称未给出]中酚类和黄酮类化合物积累以及抗氧化能力的影响。酚类和黄酮类化合物用甲醇和水性溶剂提取，并使用液相色谱 - 串联质谱法（LC - MS/MS）进行定量。咖啡酸是在[具体作物名称未给出]和[具体作物名称未给出]的水提取物中鉴定出的主要酚类化合物。芦丁是所有测试的[具体作物名称未给出]品种中含量最丰富的黄酮类化合物，在[具体品种名称未给出]中浓度最高。结果表明干旱和热胁迫对生物活性化合物积累具有强烈的正向影响，但具有物种和化合物特异性。我们得出结论，在水分充足条件下40°C的热胁迫以及干旱和热胁迫组合（30°C和35°C）似乎会诱导咖啡酸和芦丁的积累。因此，在半干旱和干旱地区种植这些品种是可行的。
