Qaddoori Malik H, Al-Shmgani Hanady S
Department of Biology, College of Education for Pure Sciences (Ibn Al-Haitham), University of Baghdad, Baghdad, Iraq.
Int J Breast Cancer. 2023 Feb 16;2023:3251211. doi: 10.1155/2023/3251211. eCollection 2023.
Angiogenesis is important for tissue during normal physiological processes as well as in a number of diseases, including cancer. Drug resistance is one of the largest difficulties to antiangiogenesis therapy. Due to their lower cytotoxicity and stronger pharmacological advantage, phytochemical anticancer medications have a number of advantages over chemical chemotherapeutic drugs. In the current study, the effectiveness of AuNPs, AuNPs-GAL, and free galangin as an antiangiogenesis agent was evaluated. Different physicochemical and molecular approaches have been used including the characterization, cytotoxicity, scratch wound healing assay, and gene expression of and in MCF-7 and MDA-MB-231 human breast cancer cell line. Results obtained from MTT assay show cell growth reduction in a time- and dose-dependent aspect; also, in comparison to individual treatment, a synergistic impact was indicated. CAM assay results demonstrated galangin-gold nanoparticle capacity to suppress angiogenesis in chick embryo. Additionally, altering and gene expression was recorded. Taken together, all the results can conclude that galangin-conjugated gold nanoparticles can be a promising antiangiogenesis supplemental drug in breast cancer treatment.
血管生成在正常生理过程以及包括癌症在内的多种疾病中对组织都很重要。耐药性是抗血管生成治疗面临的最大难题之一。由于植物化学抗癌药物具有较低的细胞毒性和更强的药理学优势，因此与化学化疗药物相比具有许多优点。在本研究中，评估了金纳米颗粒（AuNPs）、AuNPs-高良姜素（AuNPs-GAL）和游离高良姜素作为抗血管生成剂的有效性。采用了不同的物理化学和分子方法，包括表征、细胞毒性、划痕伤口愈合试验以及MCF-7和MDA-MB-231人乳腺癌细胞系中相关基因的表达。MTT试验结果显示细胞生长在时间和剂量依赖性方面有所降低；此外，与单独治疗相比，显示出协同作用。鸡胚绒毛尿囊膜（CAM）试验结果表明高良姜素-金纳米颗粒具有抑制鸡胚血管生成的能力。此外，还记录了相关基因表达的变化。综上所述，所有结果可以得出结论，高良姜素偶联金纳米颗粒可能是乳腺癌治疗中有前景的抗血管生成辅助药物。
