Department of Plastic and Aesthetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China; Department of Mechanical Engineering, University of Manitoba, 75A Chancellors Circle, Winnipeg, Manitoba R3T 2N2, Canada.
Department of Plastic and Aesthetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
J Adv Res. 2024 Jan;55:89-102. doi: 10.1016/j.jare.2023.02.014. Epub 2023 Feb 26.
Alopecia concerns more than half our adult population. Platelet-rich plasma (PRP) has been applied in skin rejuvenation and hair loss treatment. However, the pain and bleeding during injection and the troublesome for fresh preparation of each action limit PRP's in-depth applying dedication to clinics.
We report a temperature-sensitive PRP induced fibrin gel included in a detachable transdermal microneedle (MN) for hair growth.
PRP gel interpenetrated with the photocrosslinkable gelatin methacryloyl (GelMA) to realize sustained release of growth factors (GFs) and led to 14% growth in mechanical strength of a single microneedle whose strength reached 1.21 N which is sufficient to penetrate the stratum corneum. PRP-MNs' release of VEGF, PDGF, and TGF-β were characterized and quantitatively around the hair follicles (HFs) for 4-6 days consecutively. PRP-MNs promoted hair regrowth in mice models. From transcriptome sequencing, PRP-MNs induced hair regrowth through angiogenesis and proliferation. The mechanical and TGF-β sensitive gene Ankrd1 was significantly upregulated by PRP-MNs treatment.
PRP-MNs show convenient, minimally invasive, painless, inexpensive manufacture, storable and sustained effects in boosting hair regeneration.
脱发困扰着超过半数的成年人群体。富血小板血浆（PRP）已被应用于皮肤焕新和脱发治疗。然而，注射过程中的疼痛和出血，以及每次操作时 PRP 制剂的繁琐制备，限制了 PRP 在临床中的广泛应用。
我们报告了一种温度敏感的 PRP 诱导纤维蛋白凝胶，该凝胶包含在可分离的透皮微针（MN）中，用于促进头发生长。
PRP 凝胶与光交联明胶甲基丙烯酰（GelMA）互穿，实现了生长因子（GFs）的持续释放，并使单个微针的机械强度提高了 14%，其强度达到 1.21N，足以穿透角质层。PRP-MNs 在毛囊周围连续 4-6 天释放 VEGF、PDGF 和 TGF-β，并对其进行了特征描述和定量分析。PRP-MNs 促进了小鼠模型中的毛发生长。通过转录组测序，PRP-MNs 通过血管生成和增殖来诱导毛发生长。机械和 TGF-β 敏感基因 Ankrd1 经 PRP-MNs 处理后显著上调。
PRP-MNs 具有方便、微创、无痛、经济实惠、易于制备、储存和持续促进毛发生长的效果。
