Morariu Simona, Brunchi Cristina-Eliza, Honciuc Mirela, Iftime Manuela-Maria
"Petru Poni" Institute of Macromolecular Chemistry, 700487 Iasi, Romania.
Polymers (Basel). 2023 Feb 8;15(4):841. doi: 10.3390/polym15040841.
In the context of increasing interest in biomaterials with applicability in cosmetics and medicine, this research aims to obtain and characterize some hybrid materials based on chitosan (CS) (antibacterial, biocompatible, and biodegradable), poly(ethylene glycol) (PEG) (non-toxic and prevents the adsorption of protein and cell) and Laponite RD (Lap) (bioactive). The rheological properties of the starting dispersions were investigated and discussed related to the interactions developed between components. All samples exhibited gel-like properties, and the storage modulus of CS/PEG dispersion increased from 6.6 Pa to 657.7 Pa by adding 2.5% Lap. Structural and morphological characterization of the films, prepared by solution casting method, was performed by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and polarized light microscopy (POM). These analyses proved the incorporation of Lap into CS/PEG films and revealed the morphological changes of the films by the addition of clay. Thereby, at the highest Lap concentration (43.8%), the "house of cards" structure formed by Lap platelets, which incorporate chitosan chains, as evidenced by SEM and POM. Two stages of degradation between 200 °C and 410 °C were evidenced for the films with Lap concentration higher than 38.5%, explained by the existence of a clay-rich phase (given by the clay network) and chitosan-rich one (due to the intercalation of chitosan in the clay network). CS/PEG film with 43.8% Lap showed the highest swelling degree of 240.7%. The analysis of the obtained results led to the conclusion that the addition of clay to the CS/PEG films increases their stability in water and gives them greater thermal stability.
在对适用于化妆品和医学的生物材料兴趣日益浓厚的背景下，本研究旨在制备并表征一些基于壳聚糖（CS）（具有抗菌、生物相容性和可生物降解性）、聚乙二醇（PEG）（无毒且可防止蛋白质和细胞吸附）和锂皂石RD（Lap）（具有生物活性）的杂化材料。研究并讨论了起始分散体的流变性质与各组分之间形成的相互作用的关系。所有样品均表现出凝胶状性质，通过添加2.5%的Lap，CS/PEG分散体的储能模量从6.6 Pa增加到657.7 Pa。采用溶液浇铸法制备的薄膜通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、能量色散X射线光谱（EDX）和偏光显微镜（POM）进行了结构和形态表征。这些分析证明了Lap已掺入CS/PEG薄膜中，并揭示了添加粘土后薄膜的形态变化。因此，在最高Lap浓度（43.8%）下，SEM和POM证明由Lap片层形成了“纸牌屋”结构，其中包含壳聚糖链。对于Lap浓度高于38.5%的薄膜，在200℃至410℃之间观察到两个降解阶段，这可以通过存在富含粘土的相（由粘土网络形成）和富含壳聚糖的相（由于壳聚糖插入粘土网络）来解释。含有43.8% Lap的CS/PEG薄膜显示出最高的溶胀度，为240.7%。对所得结果的分析得出结论，向CS/PEG薄膜中添加粘土可提高其在水中的稳定性，并赋予它们更高的热稳定性。
