Kwong Tsz Ching, Chau Eddie Chung Ting, Mak Mark Chi Ho, Choy Chi Tung, Chan Lee Tung, Pang Chun Keung, Zhou Junwei, Poon Phoebe Hoi Ching, Guan Yuqiong, Tsui Stephen Kwok Wing, Chan Shun Wan, Leung George Pak Heng, Tai William Chi Shing, Kwan Yiu Wa
School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China.
Non-Profit Making Veterinary Services Society Limited, Hong Kong, China.
Animals (Basel). 2023 Aug 1;13(15):2479. doi: 10.3390/ani13152479.
With a close pathogenetic resemblance to human diabetes, canine Diabetes Mellitus, a chronic metabolic disease featuring abnormally high blood sugar levels, is increasing in prevalence worldwide. Unlike humans, canine glycemic control requires life-long insulin injections and dietary control in most cases, thereby jeopardizing diabetic dogs' quality of life and increasing the difficulty of disease control. While many research studies have focused on elucidating the relationship between the canine gut microbiome and diseases, there is currently no research on the subject of diabetes mellitus in dogs. We hypothesized that the gut microbiome of canines with diabetes mellitus is different from that of healthy controls. Thus, we performed targeted 16S rRNA sequencing and comprehensive bioinformatic analysis to compare the gut microbiome profiles of 16 diabetic dogs with those of 32 healthy dogs. , , , and were found to be enriched in diabetic dogs. A distinct shift towards carbohydrate degradation metabolic pathways was found to be differentially abundant in the diabetic subjects. Alteration of the co-occurrence network was also evident in the diabetic group. In conclusion, our study suggests that the gut microbial landscape differs in diabetic canines at the genera, species, functional, and network levels. These findings have significant implications for disease management, and thus warrant further research.
犬糖尿病是一种慢性代谢疾病，其特征是血糖水平异常升高，与人类糖尿病在发病机制上极为相似，且在全球范围内的患病率呈上升趋势。与人类不同的是，在大多数情况下，犬类的血糖控制需要终身注射胰岛素和进行饮食控制，这从而危及糖尿病犬的生活质量，并增加了疾病控制的难度。虽然许多研究致力于阐明犬肠道微生物群与疾病之间的关系，但目前尚无关于犬糖尿病这一主题的研究。我们推测，患有糖尿病的犬类的肠道微生物群与健康对照组不同。因此，我们进行了靶向16S rRNA测序和全面的生物信息学分析，以比较16只糖尿病犬和32只健康犬的肠道微生物群特征。发现[具体物种或菌群名称1]、[具体物种或菌群名称2]、[具体物种或菌群名称3]和[具体物种或菌群名称4]在糖尿病犬中富集。在糖尿病受试者中发现向碳水化合物降解代谢途径的明显转变存在差异丰富。糖尿病组中共同出现网络的改变也很明显。总之，我们的研究表明，在属、种、功能和网络水平上，糖尿病犬的肠道微生物景观有所不同。这些发现对疾病管理具有重要意义，因此值得进一步研究。
