Han Ya-Kang, Xu Yi-Chuang, Luo Zhi, Zhao Tao, Zheng Hua, Tan Xiao-Ying
Laboratory of Nutrition and Feed Formulation for Aquatic Economic Animals, Huazhong Agricultural University, Wuhan 430070, China.
Aquac Nutr. 2022 Nov 22;2022:2677885. doi: 10.1155/2022/2677885. eCollection 2022.
Increasing dietary replacement levels of fish meal by alternative plant proteins are of value for aquaculture. Here, a 10-week feeding experiment was undertaken to explore the effects of fish meal replacement by mixed plant protein (at a 2 : 3 ratio of cottonseed meal to rapeseed meal) on growth performance, oxidative and inflammatory responses, and mTOR pathway of yellow catfish . Yellow catfish (2.38 ± 0.1 g, mean ± SEM) were randomly divided into 15 indoors fiberglass tanks, 30 fish each tank, and fed five isonitrogenous (44% crude protein) and isolipidic (9% crude fat) diets with fish meal replaced by mixed plant protein at 0% (the control), 10% (RM10), 20% (RM20), 30% (RM30), and 40% (RM40), respectively. Among five groups, fish fed the control, and RM10 diets tended to have higher growth performance, higher protein content, and lower lipid content in livers. Dietary mixed plant protein substitute increased hepatic free gossypol content and damaged liver histology and reduced the serum total essential amino acids, total nonessential amino acids, and total amino acid contents. Yellow catfish fed the control, and RM10 diets tended to have higher antioxidant capacity. Dietary mixed plant protein replacement tended to promote proinflammatory responses and inhibited mTOR pathway. Based on the second regression analysis of SGR against mixed plant protein substitutes, the optimal replacement level of fish meal by mixed plant protein was 8.7%.
用替代植物蛋白提高鱼粉在水产养殖中的日粮替代水平具有重要价值。在此，进行了一项为期10周的饲养实验，以探究用混合植物蛋白（棉籽粕与菜籽粕比例为2∶3）替代鱼粉对黄颡鱼生长性能、氧化和炎症反应以及mTOR通路的影响。将黄颡鱼（2.38±0.1克，平均值±标准误）随机分为15个室内玻璃纤维水箱，每个水箱30尾鱼，并投喂五种等氮（粗蛋白44%）和等脂（粗脂肪9%）的日粮，鱼粉分别被混合植物蛋白以0%（对照）、10%（RM10）、20%（RM20）、30%（RM30）和40%（RM40）的比例替代。在五组中，投喂对照日粮和RM10日粮的鱼往往具有较高的生长性能、肝脏中较高的蛋白质含量和较低的脂质含量。日粮中混合植物蛋白替代物增加了肝脏游离棉酚含量，损害了肝脏组织学结构，并降低了血清总必需氨基酸、总非必需氨基酸和总氨基酸含量。投喂对照日粮和RM10日粮的黄颡鱼往往具有较高的抗氧化能力。日粮中混合植物蛋白替代物往往会促进促炎反应并抑制mTOR通路。基于特定生长率（SGR）对混合植物蛋白替代物的二次回归分析，混合植物蛋白替代鱼粉的最佳替代水平为8.7%。
