Pueyo C
Mutat Res. 1978 Dec;54(3):311-21. doi: 10.1016/0165-1161(78)90021-3.
The forward-mutation assay using the L-arabinose-sensitive strain SV3 of Salmonella typhimurium has been calibrated against a selected set of mutagens. Strain SV3 is sensitive to chemicals causing base-pair substitutions, frameshift mutations and deletions. New strains deficient for the excision-repair system or the lipopolysaccharide barrier or both have been selected from strain SV3. The additional mutations do not affect the independence of the assay from experimental artifacts due to physiological or lethal damage or differences in plating density. The new strains are more sensitive than SV3 to certain mutagens. Techniques for using this set of strains are presented and their relative advantages discussed.
利用鼠伤寒沙门氏菌的L-阿拉伯糖敏感菌株SV3进行的正向突变试验已针对一组选定的诱变剂进行了校准。菌株SV3对引起碱基对替换、移码突变和缺失的化学物质敏感。已从菌株SV3中筛选出缺乏切除修复系统或脂多糖屏障或两者都缺乏的新菌株。这些额外的突变不会影响该试验不受因生理或致死性损伤或平板接种密度差异导致的实验假象的干扰。新菌株比SV3对某些诱变剂更敏感。本文介绍了使用这组菌株的技术，并讨论了它们的相对优势。
