Department of Biological Sciences, Purdue University Fort Wayne, Fort Wayne, IN, United States of America.
PLoS One. 2023 Mar 2;18(3):e0281889. doi: 10.1371/journal.pone.0281889. eCollection 2023.
As resistance to traditional antibiotics has become a major issue, it is essential to explore natural sources for new antimicrobial agents. The marine environment offers a variety of natural bioactive compounds. In this study, we examined the antibacterial potential of Luidia clathrata, a tropical sea star species. The experiment was conducted against both gram-positive (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus and Mycobacterium smegmatis) and gram-negative (Proteus mirabilis, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) bacteria using disk diffusion method. Specifically, we extracted the body wall and gonad using methanol, ethyl acetate, and hexane. Our findings show that the body wall extract using ethyl acetate (1.78μg/ml) was particularly effective against all tested pathogens, while the gonad extract (0.107μg/ml) showed activity against six out of ten selected pathogens. This is a crucial and new discovery that suggests L. clathrata may be a useful source for discovering antibiotics and more research is required to pinpoint and comprehend the active ingredients.
随着对传统抗生素的耐药性成为一个主要问题，探索新的抗菌剂的天然来源至关重要。海洋环境提供了各种天然生物活性化合物。在这项研究中，我们检查了热带海星物种 Luidia clathrata 的抗菌潜力。该实验采用圆盘扩散法针对革兰氏阳性（枯草芽孢杆菌、粪肠球菌、金黄色葡萄球菌、蜡样芽孢杆菌和耻垢分枝杆菌）和革兰氏阴性（奇异变形杆菌、鼠伤寒沙门氏菌、大肠杆菌、铜绿假单胞菌和肺炎克雷伯菌）细菌进行。具体来说，我们使用甲醇、乙酸乙酯和正己烷提取了体壁和性腺。我们的研究结果表明，使用乙酸乙酯（1.78μg/ml）提取的体壁提取物对所有测试的病原体都特别有效，而性腺提取物（0.107μg/ml）对十种选定病原体中的六种表现出活性。这是一个重要且新的发现，表明 L. clathrata 可能是发现抗生素的有用来源，需要进一步研究以确定和理解活性成分。
