Department of Bioinformatics, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
BMC Complement Altern Med. 2010 Jan 29;10:6. doi: 10.1186/1472-6882-10-6.
The six organic solvent extracts of Artemisia nilagirica were screened for the potential antimicrobial activity against phytopathogens and clinically important standard reference bacterial strains.
The agar disk diffusion method was used to study the antibacterial activity of A. nilagirica extracts against 15 bacterial strains. The Minimum Inhibitory Concentration (MIC) of the plant extracts were tested using two fold agar dilution method at concentrations ranging from 32 to 512 microg/ml. The phytochemical screening of extracts was carried out for major phytochemical derivatives in A. nilagirica.
All the extracts showed inhibitory activity for gram-positive and gram-negative bacteria except for Klebsiella pneumoniae, Enterococcus faecalis and Staphylococcus aureus. The hexane extract was found to be effective against all phytopathogens with low MIC of 32 microg/ml and the methanol extract exhibited a higher inhibition activity against Escherichia coli, Yersinia enterocolitica, Salmonella typhi, Enterobacter aerogenes, Proteus vulgaris, Pseudomonas aeruginosa (32 microg/ml), Bacillus subtilis (64 microg/ml) and Shigella flaxneri (128 microg/ml). The phytochemical screening of extracts answered for the major derivative of alkaloids, amino acids, flavonoids, phenol, quinines, tannins and terpenoids.
All the extracts showed antibacterial activity against the tested strains. Of all, methanol and hexane extracts showed high inhibition against clinical and phytopathogens, respectively. The results also indicate the presence of major phytochemical derivatives in the A. nilagirica extracts. Hence, the isolation and purification of therapeutic potential compounds from A. nilagirica could be used as an effective source against bacterial diseases in human and plants.
研究了青蒿的 6 种有机溶剂提取物对植物病原菌和临床重要标准参考细菌菌株的潜在抗菌活性。
采用琼脂圆盘扩散法研究青蒿提取物对 15 株细菌的抗菌活性。采用两倍琼脂稀释法，在 32 至 512μg/ml 的浓度范围内测试植物提取物的最小抑菌浓度（MIC）。对提取物中的主要植物化学衍生物进行了植物化学筛选。
除肺炎克雷伯菌、粪肠球菌和金黄色葡萄球菌外，所有提取物对革兰氏阳性菌和革兰氏阴性菌均有抑制作用。正己烷提取物对所有植物病原菌均有效，MIC 为 32μg/ml，甲醇提取物对大肠杆菌、小肠结肠炎耶尔森菌、伤寒沙门氏菌、产气肠杆菌、普通变形杆菌、铜绿假单胞菌（32μg/ml）、枯草芽孢杆菌（64μg/ml）和福氏志贺菌（128μg/ml）的抑制活性较高。提取物的植物化学筛选回答了生物碱、氨基酸、类黄酮、酚类、喹啉、单宁和萜类等主要衍生物。
所有提取物均对测试菌株表现出抗菌活性。其中，甲醇和正己烷提取物对临床和植物病原菌的抑制作用最强。结果还表明，青蒿提取物中存在主要的植物化学衍生物。因此，从青蒿中分离和纯化具有治疗潜力的化合物可作为防治人类和植物细菌病的有效来源。
