Jin Yu, Sun Ruitao, Li Guangqiang, Yuan Mi, Shao Weichong, Cao Minhui, Yuan Chao, Wang Suhua
College of Science, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
College of Science, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jun 5;294:122558. doi: 10.1016/j.saa.2023.122558. Epub 2023 Feb 26.
Hydrazine (NH) can cause serious damage to human health, while intracellular viscosity is highly associated with many diseases and cellular dysfunctions. Herein, we report the synthesis of a dual-responsive organic molecule-based fluorescent probe with excellent water solubility being capable of detection of NH and viscosity through dual-fluorescence channels in "turn on" manner for both. Besides sensitive detection of NH in aqueous solution with detection limit of 0.135 μM, this probe could be used for vapor NH detection in colorimetric and fluorescent manners. In addition, the probe demonstrated viscosity-dependent fluorescence enhancement behavior, and as high as 150-fold enhancement could be obtained at 95% glycerol aqueous solution. Cell imaging experiment revealed that the probe could be used for the discriminating of living and dead cells.
肼（NH）会对人体健康造成严重损害，而细胞内粘度与许多疾病和细胞功能障碍高度相关。在此，我们报告了一种基于双响应有机分子的荧光探针的合成，该探针具有出色的水溶性，能够通过双荧光通道以“开启”方式检测NH和粘度。除了在水溶液中对NH进行灵敏检测，检测限为0.135 μM外，该探针还可用于比色和荧光方式的气态NH检测。此外，该探针表现出粘度依赖性荧光增强行为，在95%甘油水溶液中可获得高达150倍的增强。细胞成像实验表明，该探针可用于区分活细胞和死细胞。
