Li Jingbin, He Yang, Fu Jia, Wang Yimin, Fan Xing, Zhong Tian, Zhou Hui
Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Institute of Plant Resources, Dalian Minzu University, Dalian, China.
School of Life Sciences, Jilin University, Changchun, China.
Front Nutr. 2023 Feb 14;9:1121789. doi: 10.3389/fnut.2023.1121789. eCollection 2023.
extract (ASE), a dietary supplement with antifatigue, neuroprotective, and immunomodulatory properties, has been widely used due to its high polyphenol content. Our previous study showed that ASE could be used to treat Parkinson's disease (PD) as it contains multiple monoamine oxidase B inhibitors prescribed in early PD. However, its mechanism remains ambiguous. In this study, we investigated the protective effects of ASE on MPTP-induced PD in mice and explored the underlying mechanisms of action. We found that the administration of ASE significantly improved motor coordination in mice with MPTP-induced PD. As shown by quantitative proteomic analysis, 128 proteins' expression significantly changed in response to ASE administration, most of which were involved with Fcγ receptor-mediated phagocytosis in macrophages and monocytes signaling pathway, PI3K/AKT signaling pathway, and insulin receptor signaling pathway. Furthermore, the network analysis results showed that ASE modulates protein networks involved in regulating cellular assembly, lipid metabolism, and morphogenesis, all of which have implications for treating PD. Overall, ASE served as a potential therapeutic because it regulated multiple targets to improve motor deficits, which could lay the strong foundation for developing anti-PD dietary supplements.
紫锥菊提取物（ASE）是一种具有抗疲劳、神经保护和免疫调节特性的膳食补充剂，因其高多酚含量而被广泛使用。我们之前的研究表明，ASE可用于治疗帕金森病（PD），因为它含有早期PD规定使用的多种单胺氧化酶B抑制剂。然而，其作用机制仍不明确。在本研究中，我们研究了ASE对MPTP诱导的小鼠PD的保护作用，并探讨了其潜在的作用机制。我们发现，给予ASE可显著改善MPTP诱导的PD小鼠的运动协调性。定量蛋白质组学分析显示，128种蛋白质的表达在给予ASE后发生了显著变化，其中大多数与巨噬细胞和单核细胞信号通路中Fcγ受体介导的吞噬作用、PI3K/AKT信号通路和胰岛素受体信号通路有关。此外，网络分析结果表明，ASE调节参与调节细胞组装、脂质代谢和形态发生的蛋白质网络，所有这些都对治疗PD具有重要意义。总体而言，ASE作为一种潜在的治疗方法，因为它调节多个靶点以改善运动缺陷，这可为开发抗PD膳食补充剂奠定坚实基础。
