Saghrouchni Hamza, Barnossi Azeddin El, Mssillou Ibrahim, Lavkor Isilay, Ay Tahsin, Kara Mohammed, Alarfaj Abdullah A, Hirad Abdurahman Hajinur, Nafidi Hiba-Allah, Bourhia Mohammed, Var Isil
Department of Biotechnology, Institute of Natural and Applied Sciences, Çukurova University, Adana, Türkiye.
Laboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mehraz, Sidi Mohammed Ben Abdellah University, Fez, Morocco.
Front Plant Sci. 2023 Feb 10;14:973207. doi: 10.3389/fpls.2023.973207. eCollection 2023.
Perennial ryegrass ( L.) is a valuable forage and soil stabilisation crop. Perennial crops have long been associated with good environmental performance and ecosystem stability. Vascular wilt diseases caused by species are the most damaging plant diseases affecting both woody perennials and annual crops. Therefore, the aim of the present study was the assessment of the preventive and growth-promoting effects of carvacrol against , , and (phylogenetically analyzed on the basis of internal transcribed spacer (ITS) regions) causing vascular wilt of ryegrass and under greenhouse conditions. To accomplish this aim, various parameters were monitored including coleoptile development, rhizogenesis, the incidence of coleoptile lesions, disease index, the visual appearance of ryegrass health, ryegrass organic matter and soil fungal load. The results obtained showed that was highly harmful to ryegrass seedlings compared to other species. Furthermore, carvacrol with 0.1 and 0.2 mg/mL protected significantly the seedlings against Fusarium wilt diseases both and in the greenhouse. Simultaneously, carvacrol also functioned as a seedling growth promoter, as is reflected in all monitored parameters, such as the recovery of seedling height and root length, and the development of new leaf buds and secondary roots. Carvacrol proved to be effective plant growth promoter and a bio-fungicide against Fusarium vascular diseases.
多年生黑麦草（Lolium perenne L.）是一种重要的饲料和土壤固持作物。多年生作物长期以来一直与良好的环境表现和生态系统稳定性相关联。由尖孢镰刀菌（Fusarium oxysporum）、茄病镰刀菌（F. solani）和轮枝镰刀菌（F. verticillioides）（基于内转录间隔区（ITS）区域进行系统发育分析）引起的维管束枯萎病是影响木本多年生植物和一年生作物的最具破坏性的植物病害。因此，本研究的目的是评估香芹酚对引起黑麦草维管束枯萎病的尖孢镰刀菌、茄病镰刀菌和轮枝镰刀菌在温室条件下的预防和促生长作用。为实现这一目标，监测了各种参数，包括胚芽鞘发育、发根、胚芽鞘病变发生率、病情指数、黑麦草健康状况的外观、黑麦草有机质和土壤真菌载量。获得的结果表明，与其他镰刀菌物种相比，尖孢镰刀菌对黑麦草幼苗危害极大。此外，0.1和0.2 mg/mL的香芹酚在温室中均能显著保护幼苗免受镰刀菌枯萎病的侵害。同时，香芹酚还起到了幼苗生长促进剂的作用，这在所有监测参数中都有体现，如幼苗高度和根长的恢复以及新叶芽和次生根的发育。香芹酚被证明是一种有效的植物生长促进剂和抗镰刀菌维管束病害的生物杀菌剂。
